Protein dynamics in solution and powder measured by incoherent elastic neutron scattering: the influence of Q-range and energy resolution

European Biophysics Journal : EBJ
Frank Gabel

Abstract

Incoherent elastic neutron scattering (IENS) has been widely used to measure intramolecular atomic mean square displacements (MSDs) of proteins in powder and in solution. The instrumental energy resolution and the wave vector transfer (Q-range) determine, respectively, the time and length scales of observable motions. In order to investigate contributions of diffusive motions to MSDs measured by this method, we calculated the elastic intensity for several simple scattering functions. We showed that continuous translational diffusion contributes to MSDs in a Q-range where the energy width of the scattering function is of the order of the instrumental energy resolution. We discuss the choice of instruments adapted to focus on intramolecular motions in the presence of solvent or global macromolecular diffusion. The concepts developed are applied to interpret experimental data from H(2)O- and D(2)O-hydrated proteins. Finally, analogies between the Gaussian approximation in IENS and the Guinier approximation in small-angle scattering are discussed.

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Citations

Jun 14, 2005·Biochimica Et Biophysica Acta·Moeava Tehei, Giuseppe Zaccai
Apr 7, 2007·The Journal of Chemical Physics·Gerald R Kneller, Vania Calandrini
Nov 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·K WoodM Weik
Jul 27, 2006·European Biophysics Journal : EBJ·Moeava TeheiGiuseppe Zaccai
Feb 14, 2008·European Biophysics Journal : EBJ·Wolfgang Doster
Feb 21, 2008·European Biophysics Journal : EBJ·Marion JasninGiuseppe Zaccai
May 31, 2012·European Biophysics Journal : EBJ·Giuseppe Zaccai
Dec 18, 2015·The Journal of Physical Chemistry. B·Veerendra Kumar SharmaVolker S Urban
Sep 16, 2016·The Journal of Physical Chemistry Letters·Utsab R ShresthaXiang-Qiang Chu
Dec 30, 2014·Journal of Intellectual Disabilities : JOID·Kristín BjörnsdóttirÁstríður Stefánsdóttir
May 16, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Daniela RussoJohn R D Copley
Jul 23, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Salvatore MagazùAntonio Benedetto
Oct 6, 2005·The Journal of Biological Chemistry·Moeava TeheiGiuseppe Zaccai
Apr 4, 2017·International Journal of Pharmaceutics·Murillo L MartinsHeloisa N Bordallo
Nov 7, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·S O DialloE Mamontov
Nov 29, 2012·The Journal of Physical Chemistry. B·Marcus TrappJudith Peters
Jul 19, 2012·The Journal of Physical Chemistry. B·Xiang-qiang ChuLeighton Coates

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