Protein Engineering for Nicotinamide Coenzyme Specificity in Oxidoreductases: Attempts and Challenges

Frontiers in Microbiology
Andrea M Chánique, Loreto P Parra

Abstract

Oxidoreductases are ubiquitous enzymes that catalyze an extensive range of chemical reactions with great specificity, efficiency, and selectivity. Most oxidoreductases are nicotinamide cofactor-dependent enzymes with a strong preference for NADP or NAD. Because these coenzymes differ in stability, bioavailability and costs, the enzyme preference for a specific coenzyme is an important issue for practical applications. Different approaches for the manipulation of coenzyme specificity have been reported, with different degrees of success. Here we present various attempts for the switching of nicotinamide coenzyme preference in oxidoreductases by protein engineering. This review covers 103 enzyme engineering studies from 82 articles and evaluates the accomplishments in terms of coenzyme specificity and catalytic efficiency compared to wild type enzymes of different classes. We analyzed different protein engineering strategies and related them with the degree of success in inverting the cofactor specificity. In general, catalytic activity is compromised when coenzyme specificity is reversed, however when switching from NAD to NADP, better results are obtained. In most of the cases, rational strategies were used, predominantly with ...Continue Reading

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Citations

Sep 29, 2018·ChemSusChem·Fabio ToninIsabel W C E Arends
Sep 25, 2018·Protein Science : a Publication of the Protein Society·Jacob BallGiovanni Gadda
Mar 17, 2020·Chembiochem : a European Journal of Chemical Biology·Yuxue LiuZongbao K Zhao
Apr 20, 2021·Frontiers in Bioengineering and Biotechnology·Gamal Nasser Abdel-HadyRyuichi Hirota
Mar 16, 2021·Angewandte Chemie·Ioannis ZachosVolker Sieber
May 27, 2021·World Journal of Microbiology & Biotechnology·Kyung-Chul Shin, Deok-Kun Oh
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Aug 1, 2020·ACS Catalysis·Liliana Calzadiaz-RamirezCarlos G Acevedo-Rocha
Nov 26, 2021·Chemical Communications : Chem Comm·Suphanida WorakaensaiRung-Yi Lai

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