Protein Expression and Purification of the Hsp90-Cdc37-Cdk4 Kinase Complex from Saccharomyces cerevisiae.

Bio-protocol
Kliment A Verba, David A Agard

Abstract

Interactions between Hsp90, its co-chaperone Cdc37 and kinases have been biochemically studied for over three decades and have been shown to be functionally important in organisms from yeast to humans. However, formation of a stable complex for structural studies has been elusive. In this protocol we describe expression and purification of Hsp90-Cdc37-Cdk4 kinase protein complex from Saccharomyces cerevisiae utilizing the viral 2A sequences to titrate the three proteins at similar levels.

References

Sep 5, 2006·Molecular Cell·Cara K VaughanLaurence H Pearl
Jul 24, 2013·BioMed Research International·Ekaterina Minskaia, Martin D Ryan

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