PMID: 16522181Mar 9, 2006Paper

Protein expression, crystallization and preliminary X-ray crystallographic studies of YjbK from Bacillus subtilis

Protein and Peptide Letters
Xueyu DaiXiao-Dong Su

Abstract

B. subtilis YjbK is a protein with 190 residues of uncharacterized function, it has been annotated by Pfam database as a member of adenylate cyclase family (EC: 4.6.1.1). In order to identify its exact function via structural studies, yjbK gene was amplified from B. subtilis genomic DNA and cloned into expression vector pET21-DEST. The protein was expressed in a soluble form in E. coli and purified to homogeneity. YjbK was crystallized and diffracted to a resolution of 2.0 A in-house. The crystals belong to P1 space group, with unit cell parameters a = 32.38 A, b = 34.69 A, c = 46.02 A, alpha = 96.560 degrees, beta = 99.683 degrees, gamma = 111.333 degrees. There is one molecule per asymmetric unit.

Citations

Nov 28, 2009·Molecular and Cellular Biochemistry·Jürgen U Linder

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