Protein expression differs between neural progenitor cells from the adult rat brain subventricular zone and olfactory bulb

BMC Neuroscience
Martin H MaurerWolfgang Kuschinsky

Abstract

Neural progenitor cells can be isolated from various regions of the adult mammalian brain, including the forebrain structures of the subventricular zone and the olfactory bulb. Currently it is unknown whether functional differences in these progenitor cell populations can already be found on the molecular level. Therefore, we compared protein expression profiles between progenitor cells isolated from the subventricular zone and the olfactory bulb using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry. The subventricular zone and the olfactory bulb are connected by the Rostral Migratory Stream (RMS), in which glial fibrillary acidic protein (GFAP)-positive cells guide neuroblasts. Recent literature suggested that these GFAP-positive cells possess neurogenic potential themselves. In the current study, we therefore compared the cultured neurospheres for the fraction of GFAP-positive cells and their morphology of over a prolonged period of time. We found significant differences in the protein expression patterns between subventricular zone and olfactory bulb neural progenitor cells. Of the differentially expressed protein spots, 105 were exclusively expressed in the subventricular zone, 23 sho...Continue Reading

References

Jul 1, 1982·Journal of Neurochemistry·M Schachner
May 20, 1994·Science·C Lois, A Alvarez-Buylla
May 1, 1997·Neuron·H WichterleA Alvarez-Buylla
Aug 26, 1998·Journal of Neurobiology·J M García-VerdugoA Alvarez-Buylla
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·D R Kornack, P Rakic
Nov 19, 2002·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J R L MenezesC Hedin-Pereira
May 23, 2003·Glia·Arnold R Kriegstein, Magdalena Götz
Jun 24, 2003·Proteome Science·Martin H MaurerWolfgang Kuschinsky
Oct 30, 2003·Nature Neuroscience·Fiona Doetsch
May 18, 2004·Neurochemistry International·Harold K Kimelberg
Sep 2, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lei WangMichael Chopp
Oct 26, 2005·Current Topics in Developmental Biology·Tetsuji MoriMagdalena Götz
Nov 30, 2005·Nature Reviews. Molecular Cell Biology·Magdalena Götz, Wieland B Huttner
Nov 7, 2006·Journal of Theoretical Biology·Nebojsa T Milosević, Dusan Ristanović
Jul 7, 2007·Science·Florian T MerkleArturo Alvarez-Buylla
Jan 29, 2008·Current Stem Cell Research & Therapy·Martin H Maurer, Wolfgang Kuschinsky
Sep 17, 2008·Genes & Development·Hai-Kun LiuGünther Schütz

❮ Previous
Next ❯

Citations

Apr 4, 2009·Proteomics·Devendra Kumar MauryaPurnima Bhargava
Sep 18, 2008·Journal of Neuroscience Research·Massimo GulisanoGabriella B Vannelli
Aug 2, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Sara Gil-PerotínJosé Manuel García-Verdugo
Oct 17, 2009·Proteomics·Purnima Bhargava
Aug 18, 2017·Omics : a Journal of Integrative Biology·Manjunath DammalliThottethodi Subrahmanya Keshava Prasad
Aug 21, 2009·Nature Reviews. Neuroscience·Alex Bayés, Seth G N Grant
Nov 20, 2012·Electrophoresis·Martin H Maurer
Jul 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·S L BernsteinZ Mehrabian
Oct 9, 2019·Aging and Disease·Brian Z WangKunlin Jin

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
fluorescence microscopy
dissection

Software Mentioned

SPSS
TissueQuest
MCID Elite
Mascot
Phoretix

Related Concepts

Related Feeds

Astrocytes in Repair & Regeneration

Astrocytes are glial cells found within the CNS and are able to regenerate new neurons. They become activated during CNS injury and disease. The activation leads to the transcription of new genes and the repair and regeneration of neurons. Discover the latest research on astrocytes in repair and regeneration here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.