Protein expression profiling of postmortem brain in schizophrenia

Schizophrenia Research
Jianfeng MeiBarbara Porton

Abstract

Surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) enables the sensitive, high-throughput protein profiling of complex biological mixtures. In combination with bioinformatics, this technology has the potential to identify combinations of spectral peaks that can differentiate individuals with a particular disease from normal controls. SELDI-TOF-MS was used to screen postmortem tissue derived from the dorsolateral prefrontal cortex of individuals with schizophrenia (n = 34) and matched controls (n = 35), obtained from the Stanley Foundation Neuropathology Consortium. Tissue samples were homogenized in urea buffer, applied to four different chip arrays which possess different chromatographic surfaces, and analyzed using the Ciphergen ProteinChip Biomarkers System (Model PBS II). Protein expression profiles of the schizophrenia and control groups were compared and analyzed using the Ciphergen Express (CE) and Biomarker Patterns Software (BPS) package. We detected several protein peaks whose intensities differed between the schizophrenia and control groups to a highly significant degree. A combination of these peaks was capable of distinguishing between schizophrenia and controls with a sens...Continue Reading

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Citations

Jan 1, 2011·Schizophrenia Research and Treatment·Brian Dean
Jul 19, 2006·Diagnostic Pathology·Shaheen Emmanuel Lakhan
Feb 26, 2016·Frontiers in Cellular Neuroscience·Katarina DavalievaAndrew J Dwork
Aug 17, 2010·Neurochemistry International·Rita MachaalaniMaurice Dematteis
Feb 7, 2015·Cell Biochemistry and Biophysics·Yin-Huan DingKai-Zheng Wang
Feb 10, 2010·Journal of Psychopharmacology·Regina TaurinesJohannes Thome
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May 10, 2008·Expert Review of Proteomics·Haruka Matsumoto, Izuru Matsumoto

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