PMID: 8939629Oct 1, 1996Paper
Protein expression using ubiquitin fusion and cleavage
Current Opinion in Biotechnology
R T Baker
Abstract
Expressing proteins and polypeptides as fusions to ubiquitin offers the advantage of an often dramatic increase in yield, and the ability to produce any desired amino-terminal residue upon ubiquitin cleavage. The recent availability of cloned ubiquitin-cleaving enzymes has enhanced this technique for both bacterial and eukaryotic host systems.
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