PMID: 8939629Oct 1, 1996Paper

Protein expression using ubiquitin fusion and cleavage

Current Opinion in Biotechnology
R T Baker

Abstract

Expressing proteins and polypeptides as fusions to ubiquitin offers the advantage of an often dramatic increase in yield, and the ability to produce any desired amino-terminal residue upon ubiquitin cleavage. The recent availability of cloned ubiquitin-cleaving enzymes has enhanced this technique for both bacterial and eukaryotic host systems.

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Citations

Apr 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sylvestre MarillonnetYuri Gleba
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