Protein extraction from mature rice leaves for two-dimensional gel electrophoresis and its application in proteome analysis

Proteomics
Nazrul IslamR Akhurst

Abstract

Sample preparation is crucial for extraction and higher resolution of proteins by two-dimensional gel electrophoresis (2-DE). In this study, we present an efficient protocol to extract proteins from mature rice leaves by minimizing the presence of nonprotein contaminants and by maximizing contact between the sample and extraction buffer. A combination of chemical and physical processes remarkably improved protein extraction for 2-DE. The efficiency of this protocol was demonstrated by comparison of the rice proteome at two developmental stages.

Citations

Mar 9, 2010·Plant & Cell Physiology·Keiichi Mochida, Kazuo Shinozaki
Apr 9, 2013·Journal of Proteomics·Mark V BoggessKumar Bala
Sep 8, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anne-Christine SchmidtBianca Störr
Nov 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna Bodzon-KulakowskaJerzy Silberring
Aug 3, 2005·Proteomics·Christine R FaulknerRobyn L Overall
Jun 16, 2005·Mass Spectrometry Reviews·Ganesh Kumar Agrawal, Randeep Rakwal
Jun 23, 2006·Proteomics·Cristina-Maria Vâlcu, Katja Schlink
Jul 20, 2007·Proteomics·Ghasem Hosseini Salekdeh, Setsuko Komatsu
Jun 4, 2013·Biotechnology and Applied Biochemistry·Daryush TaleiMohd Abdullah Puad
Oct 31, 2014·Applied Biochemistry and Biotechnology·Aphinya WongpiaHataichanoke Niamsup
Oct 25, 2016·Journal of Proteomics·Bertrand ColignonSergio Mauro
Jul 21, 2017·Molecular Plant-microbe Interactions : MPMI·Bertrand ColignonSergio Mauro
Jan 15, 2017·Journal of Chromatographic Science·Bilal AslamMuhammad Hidayat Rasool
Sep 2, 2020·Analytical Biochemistry·Nazrul IslamSavithiry Natarajan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.