PMID: 2491328Jan 1, 1989Paper

Protein extraction with reverse micelles

Chinese Journal of Biotechnology
H Q ShiZ Y Shen

Abstract

Liquid-liquid protein extraction with reverse micelles is a new method for protein separation. We studied the effects of pH, ionic concentration, cation type, and protein molecular mass on performance of an AOT/isooctane reverse micelle system in extraction of lysozyme, trypsin, and pepsin. Extraction was found to be very efficient for low-molecular-mass proteins.

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