Protein film voltammetry of arsenite oxidase from the chemolithoautotrophic arsenite-oxidizing bacterium NT-26

Biochemistry
Paul V Bernhardt, Joanne M Santini

Abstract

The chemolithoautotrophic bacterium NT-26 (isolated from a gold mine in the Northern Territory of Australia) is unusual in that it acquires energy by oxidizing arsenite to arsenate while most other arsenic-oxidizing organisms perform this reaction as part of a detoxification mechanism against the potentially harmful arsenite [present as As(OH)(3) at neutral pH]. The enzyme that performs this reaction in NT-26 is the molybdoenzyme arsenite oxidase, and it has been previously isolated and characterized. Here we report the direct (unmediated) electrochemistry of NT-26 arsenite oxidase confined to the surface of a pyrolytic graphite working electrode. We have been able to demonstrate that the enzyme functions natively while adsorbed on the electrode where it displays stable and reproducible catalytic electrochemistry in the presence of arsenite. We report a pH dependence of the catalytic electrochemical potential of -33 mV/pH unit that is indicative of proton-coupled electron transfer. We also have performed catalytic voltammetry at a number of temperatures between 5 and 25 degrees C, and the catalytic current (proportional to the turnover number) follows simple Arrhenius behavior.

References

Nov 7, 1996·Chemical Reviews·Russ Hille
Jun 22, 2002·Toxicology Letters·Michael F Hughes
Sep 19, 2002·Journal of the American Chemical Society·Thomas ConradsRuss Hille
Dec 18, 2002·Journal of Bacteriology·Daniel MullerMarie-Claire Lett
Mar 5, 2004·Journal of Bacteriology·Joanne M Santini, Rachel N vanden Hoven

❮ Previous
Next ❯

Citations

Nov 15, 2007·Chemical Reviews·My Hang V Huynh, Thomas J Meyer
Jun 19, 2012·Chemical Reviews·David R WeinbergThomas J Meyer
Jan 29, 2014·Chemical Reviews·Russ HillePartha Basu
Dec 17, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kuan-I ChenPaul V Bernhardt
Jun 14, 2013·Environmental Microbiology Reports·Djamila Slyemi, Violaine Bonnefoy
Oct 19, 2011·Biochemical and Biophysical Research Communications·Philippa J L Simpson, Rachel Codd
Sep 18, 2012·Biochimica Et Biophysica Acta·Robert van LisBarbara Schoepp-Cothenet
Feb 20, 2007·Biochimica Et Biophysica Acta·Joanne M SantiniPaul V Bernhardt
Dec 28, 2010·Chemical Communications : Chem Comm·Paul V Bernhardt
Sep 1, 2012·The Journal of Physical Chemistry. B·Palraj KalimuthuPaul V Bernhardt
Feb 12, 2017·Journal of Industrial Microbiology & Biotechnology·Van Khanh NguyenTaeho Lee
Aug 7, 2007·The Journal of Physical Chemistry. B·Patrick BertrandChristophe Léger
Jul 16, 2008·Chemical Reviews·Christophe Léger, Patrick Bertrand

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.