Protein fingerprinting: a novel virus identification system

Journal of Virological Methods
P WalpitaD Pfeifer

Abstract

Viral proteins separated by one-dimensional SDS-PAGE produce protein binding patterns (fingerprints) which are unique for different viruses. We have applied this concept successfully for the development of a practical and objective virus identification system which is applicable to most viruses. The method is simple, specific, and, unlike the currently available methods, free from all virus-specific reagents. Interference by host protein bands in SDS-PAGE preparations of virus-infected cell lysates was eliminated consistently by treating virus infected cell cultures with optimum concentration of NaCl for selective inhibition of host protein synthesis. The method utilizes the comparison of protein fingerprints of 'unknown' viruses with protein fingerprints of reference viruses stored in a computer data base, using pattern recognition software. All 113 'unknown' virus strains were correctly identified to the genus level by the protein fingerprint method, when compared with the conventional virus identification methods.

References

Oct 1, 1977·The Journal of General Virology·S LevineR Hamilton
Jun 1, 1989·Journal of Virological Methods·J D Connor, P Walpita
Jan 1, 1986·Intervirology·V Zaslavsky, P H Hofschneider
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M Kozak
May 15, 1974·Journal of Molecular Biology·J L SaborioG Koch

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Citations

Jun 10, 1998·Journal of Clinical Microbiology·D T HollandJ D Connor
Apr 1, 1992·Journal of Clinical Microbiology·P WalpitaJ D Connor

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