Protein-folding kinetics and mechanisms studied by pulse-labeling and mass spectrometry

Mass Spectrometry Reviews
Lars Konermann, Douglas A Simmons

Abstract

The "protein-folding problem" refers to the question of how and why a denatured polypeptide chain can spontaneously fold into a compact and highly ordered conformation. The classical description of this process in terms of reaction pathways has been complemented by models that describe folding as a biased conformational diffusion on a multidimensional energy landscape. The identification and characterization of short-lived intermediates provide important insights into the mechanism of folding. Pulsed hydrogen/deuterium exchange (HDX) methods are among the most powerful tools for studying the properties of kinetic intermediates. Analysis of pulse-labeled proteins by mass spectrometry (MS) provides information that is complementary to that obtained in nuclear magnetic resonance (NMR) studies; NMR data represent an average of entire protein ensembles, whereas MS can detect co-existing protein species. MS-based pulse-labeling experiments can distinguish between folding scenarios that involve parallel pathways, and those where folding is channeled through obligatory intermediates. The proteolytic digestion/MS technique provides spatially resolved information on the HDX pattern of folding intermediates. This method is especially impo...Continue Reading

References

Mar 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T E Creighton
Apr 1, 1991·Rapid Communications in Mass Spectrometry : RCM·V Katta, B T Chait
Dec 13, 1991·Science·H FrauenfelderP G Wolynes
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J B Udgaonkar, R L Baldwin
Jul 20, 1973·Science·C B Anfinsen
Jan 1, 1966·Advances in Protein Chemistry·A Hvidt, S O Nielsen
Nov 1, 1983·Quarterly Reviews of Biophysics·S W Englander, N R Kallenbach
Oct 29, 1982·Molecular and Cellular Biochemistry·C WoodwardE Tüchsen
Sep 1, 1995·Trends in Biochemical Sciences·O B Ptitsyn
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·T Kiefhaber
Jul 14, 1995·Science·Y BaiS W Englander
Mar 1, 1994·Nature Structural Biology·T E Creighton
Mar 1, 1994·Nature Structural Biology·T R SosnickS W Englander
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·J N OnuchicN D Socci
Dec 1, 1994·Protein Science : a Publication of the Protein Society·R S Johnson, K A Walsh
May 30, 1995·Biochemistry·B B KragelundF M Poulsen
Apr 1, 1995·Nature Structural Biology·L C WuP S Kim
Sep 1, 1993·Proteins·Y BaiS W Englander
Sep 1, 1993·Proteins·G P ConnellyS W Englander
Nov 5, 1993·Science·S W Englander
Sep 1, 1995·Protein Science : a Publication of the Protein Society·D W Miller, K A Dill
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A MirankerC M Dobson
Nov 1, 1995·Bioconjugate Chemistry·J A Loo
Jul 1, 1996·Nature Structural Biology·M Jamin, R L Baldwin
Feb 1, 1996·Current Opinion in Structural Biology·S W EnglanderL Mayne
Jan 1, 1997·Nature Structural Biology·K A Dill, H S Chan
Jan 1, 1997·Trends in Biochemical Sciences·T E Creighton

❮ Previous
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Citations

Oct 22, 2011·Journal of the American Society for Mass Spectrometry·Anastasia KharlamovaScott A McLuckey
Sep 21, 2012·Journal of the American Society for Mass Spectrometry·Hong LinJohn S Klassen
Dec 14, 2004·Journal of the American Society for Mass Spectrometry·Richard A Ochran, Lars Konermann
Aug 29, 2006·Journal of the American Society for Mass Spectrometry·Rinat R Abzalimov, Igor A Kaltashov
Dec 19, 2006·Journal of the American Society for Mass Spectrometry·Nick C PolferJos Oomens
Jul 3, 2007·Journal of the American Society for Mass Spectrometry·Peter W MurphyDavid M Byers
May 15, 2003·Journal of the American Society for Mass Spectrometry·Sonya M Clark, Lars Konermann
Aug 31, 2006·Journal of the American Chemical Society·Stormy L KoenigerDavid E Clemmer
Mar 25, 2010·Molecular & Cellular Proteomics : MCP·Ilya GertsmanJohn E Johnson
Dec 1, 2012·Molecular & Cellular Proteomics : MCP·Kyle M BurnsDavid C Schriemer
Jun 25, 2008·Annual Review of Biophysics·Jayant B Udgaonkar
Jan 5, 2014·Antioxidants & Redox Signaling·Chad R Borges, Nisha D Sherma
Sep 1, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Maria Elisa Crestoni, Simonetta Fornarini
Dec 20, 2008·Journal of the American Society for Mass Spectrometry·Hui-Min ZhangAlan G Marshall
Aug 16, 2005·Expert Review of Proteomics·Sonya M SchermannLars Konermann
Mar 13, 2012·Analytica Chimica Acta·Andrew J PercyDavid C Schriemer
Jun 30, 2009·Methods : a Companion to Methods in Enzymology·Anne E Bunner, James R Williamson
Dec 29, 2007·Analytical Biochemistry·Pegah R Jalili, Chhabil Dass
Jul 16, 2005·Proteins·Alessandro Pintar, Sándor Pongor
Feb 4, 2005·Protein Science : a Publication of the Protein Society·Wolfgang RistMatthias P Mayer
Apr 21, 2004·Protein Science : a Publication of the Protein Society·Ewald T J van den BremerAlbert J R Heck
Feb 21, 2007·Rapid Communications in Mass Spectrometry : RCM·Gaetano Invernizzi, Rita Grandori
Mar 15, 2006·Annals of the New York Academy of Sciences·Weihua GuoR Stephen Berry
Feb 26, 2009·Mass Spectrometry Reviews·Nick C Polfer, Jos Oomens
Aug 13, 2009·Mass Spectrometry Reviews·Lars KonermannXin Tong
Jul 28, 2011·Angewandte Chemie·Charlotte Uetrecht, Albert J R Heck
Jul 12, 2011·Angewandte Chemie·Kalyaneswar Mandal, Stephen B H Kent
Jun 11, 2014·Journal of Mass Spectrometry : JMS·Anastasia KharlamovaScott A McLuckey
Aug 30, 2005·Current Opinion in Chemical Biology·Jonas BorchPeter Roepstorff
Nov 16, 2005·Proteins·Véronique Receveur-BréchotSonia Longhi
Aug 16, 2015·Biophysical Journal·Srinath KrishnamurthyGanesh S Anand

❮ Previous
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