Protein imaging. Single-protein spin resonance spectroscopy under ambient conditions

Science
Fazhan ShiJiangfeng Du

Abstract

Magnetic resonance is essential in revealing the structure and dynamics of biomolecules. However, measuring the magnetic resonance spectrum of single biomolecules has remained an elusive goal. We demonstrate the detection of the electron spin resonance signal from a single spin-labeled protein under ambient conditions. As a sensor, we use a single nitrogen vacancy center in bulk diamond in close proximity to the protein. We measure the orientation of the spin label at the protein and detect the impact of protein motion on the spin label dynamics. In addition, we coherently drive the spin at the protein, which is a prerequisite for studies involving polarization of nuclear spins of the protein or detailed structure analysis of the protein itself.

References

Mar 15, 2000·Protein Science : a Publication of the Protein Society·V GaponenkoP R Rosevear
Mar 17, 2001·Science·P P BorbatJ H Freed
Oct 4, 2008·Nature·Gopalakrishnan BalasubramanianJörg Wrachtrup
Apr 8, 2009·Nature Materials·Gopalakrishnan BalasubramanianJörg Wrachtrup
Dec 21, 2011·Physical Review Letters·Marcus SchaffrySimon C Benjamin
Mar 23, 2012·Nature·M C ScottJianwei Miao
Apr 25, 2013·Nature Communications·N Bar-GillR L Walsworth
Nov 26, 2013·Nature·Thomas R M BarendsIlme Schlichting
Aug 26, 2014·Nature Communications·C MüllerF Jelezko
Oct 22, 2014·Nano Letters·A O SushkovM D Lukin
Nov 22, 2014·Physical Review Letters·A O SushkovM D Lukin
Dec 23, 2014·Nature Nanotechnology·Marc Cahay

❮ Previous
Next ❯

Citations

Mar 7, 2015·Science·Philip Hemmer, Carmen Gomes
Feb 26, 2016·Nanoscale·Junfeng WangGuanzhong Wang
Nov 14, 2015·Journal of the American Chemical Society·Yongqiang FengChunru Wang
Jun 27, 2015·Small·Alberto BorettiStefania Castelletto
Apr 28, 2016·Angewandte Chemie·Yuzhou WuTanja Weil
May 12, 2016·Biophysical Journal·Richelle M Teeling-SmithP Chris Hammel
Jul 1, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Jiamei CaoHongguang Li
Jul 6, 2016·Journal of Magnetic Resonance·Jörg Wrachtrup, Amit Finkler
Sep 16, 2015·Scientific Reports·Andrii Lazariev, Gopalakrishnan Balasubramanian
May 18, 2017·Nature Communications·Felipe Fávaro de OliveiraJörg Wrachtrup
Jul 4, 2017·Nature Communications·James D A WoodLloyd C L Hollenberg
Aug 19, 2017·Science Advances·Lukas SchlipfAmit Finkler
Sep 8, 2017·Nature Communications·David A SimpsonLloyd C L Hollenberg
Jan 1, 2018·The Review of Scientific Instruments·J L PachecoE Bielejec
Nov 25, 2017·Physical Chemistry Chemical Physics : PCCP·Mihajlo NovakovicLucio Frydman
Apr 21, 2018·Nature Communications·Fei KongJiangfeng Du
Sep 1, 2018·Light, Science & Applications·Martina BarbieroMin Gu
Jul 16, 2020·Advanced Materials·Xiaojing XiaPeter J Pauzauskie
Aug 28, 2019·Nature Protocols·Dominik B BucherRonald L Walsworth
Aug 21, 2020·Advanced Materials·Kang LiuJiaqi Zhu
Nov 5, 2016·Reports on Progress in Physics·Wen YangRen-Bao Liu
Feb 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ashok AjoyPaola Cappellaro
Aug 8, 2018·Nature Methods·Fazhan ShiJiangfeng Du
Jun 8, 2019·Physical Review Letters·Zhen-Yu WangMartin B Plenio
Apr 24, 2019·Physical Review Letters·A AjoyP Cappellaro
Apr 4, 2017·Nanotechnology·K Turcheniuk, Vadym N Mochalin
Jun 6, 2017·Physical Review Letters·Haibin LiuJianming Cai
May 6, 2017·Physical Review Letters·Scott E LillieLloyd C L Hollenberg
Aug 3, 2018·The Review of Scientific Instruments·Xi QinJiang-Feng Du
Apr 17, 2019·Science Advances·Pengfei WangJiangfeng Du
Mar 9, 2019·Physical Review Letters·Dolev BluvsteinAnia C Bleszynski Jayich
Jul 9, 2020·Physical Review Letters·Rui LiJiangfeng Du
Nov 30, 2019·The Review of Scientific Instruments·Wen-Zhe ZhangJiang-Feng Du
Feb 6, 2017·Physical Review Letters·Florian M HrubeschMartin S Brandt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.