Protein interaction profiling of the p97 adaptor UBXD1 points to a role for the complex in modulating ERGIC-53 trafficking.

Molecular & Cellular Proteomics : MCP
Dale S HainesRaymond J Deshaies

Abstract

UBXD1 is a member of the poorly understood subfamily of p97 adaptors that do not harbor a ubiquitin association domain or bind ubiquitin-modified proteins. Of clinical importance, p97 mutants found in familial neurodegenerative conditions Inclusion Body Myopathy Paget's disease of the bone and/or Frontotemporal Dementia and Amyotrophic Lateral Sclerosis are defective at interacting with UBXD1, indicating that functions regulated by a p97-UBXD1 complex are altered in these diseases. We have performed liquid chromatography-mass spectrometric analysis of UBXD1-interacting proteins to identify pathways in which UBXD1 functions. UBXD1 displays prominent association with ERGIC-53, a hexameric type I integral membrane protein that functions in protein trafficking. The UBXD1-ERGIC-53 interaction requires the N-terminal 10 residues of UBXD1 and the C-terminal cytoplasmic 12 amino acid tail of ERGIC-53. Use of p97 and E1 enzyme inhibitors indicate that complex formation between UBXD1 and ERGIC-53 requires the ATPase activity of p97, but not ubiquitin modification. We also performed SILAC-based quantitative proteomic profiling to identify ERGIC-53 interacting proteins. This analysis identified known (e.g. COPI subunits) and novel (Rab3GAP...Continue Reading

References

Nov 24, 1999·Nature Cell Biology·C AppenzellerH P Hauri
Jan 5, 2002·Molecular and Cellular Biology·Efrat RabinovichShoshana Bar-Nun
Mar 8, 2005·Journal of Biochemistry·Keiji Uchiyama, Hisao Kondo
Apr 18, 2006·Cell·Leonard J FosterMatthias Mann
Jan 23, 2008·Biochemical Society Transactions·Heidi O YeungPaul S Freemont
Jan 23, 2008·Biochemical Society Transactions·Hemmo Meyer, Oliver Popp
Feb 20, 2008·The Journal of Cell Biology·Beat NyfelerHans-Peter Hauri
Apr 29, 2008·Cellular and Molecular Life Sciences : CMLS·C Schuberth, A Buchberger
Jul 29, 2008·The International Journal of Biochemistry & Cell Biology·Louise MadsenRasmus Hartmann-Petersen
Sep 9, 2008·Cell·Gabriela AlexandruRaymond J Deshaies
Jun 6, 2009·The International Journal of Biochemistry & Cell Biology·Louise MadsenRasmus Hartmann-Petersen
Jul 15, 2009·Seminars in Thrombosis and Hemostasis·Marta Spreafico, Flora Peyvandi
Dec 17, 2009·The Journal of Cell Biology·Jeong-Sun JuConrad C Weihl
Dec 23, 2009·Hybridoma·Poornima RamkumarDale S Haines
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Miho NishioKoichi Kato
Apr 24, 2010·EMBO Reports·Vanesa Fernández-Sáiz, Alexander Buchberger
Dec 15, 2010·Neuron·Janel O JohnsonBryan J Traynor
Dec 21, 2010·Molecular & Cellular Proteomics : MCP·J Eugene LeeRaymond J Deshaies
Jan 25, 2011·Journal of Proteome Research·Jürgen CoxMatthias Mann
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tsui-Fen ChouRaymond J Deshaies
Jul 26, 2011·Biochimica Et Biophysica Acta·Kunitoshi YamanakaTeru Ogura
Aug 3, 2011·Biochimica Et Biophysica Acta·Catherine Dargemont, Batool Ossareh-Nazari
Sep 29, 2011·Biochimica Et Biophysica Acta·Dieter H Wolf, Alexandra Stolz

❮ Previous
Next ❯

Citations

Oct 12, 2013·Reviews in Endocrine & Metabolic Disorders·Jonathan P BelmanJonathan S Bogan
Nov 14, 2015·Autophagy·Andreas KernChristian Behl
Aug 13, 2015·Biochemical Pharmacology·Yanfen TengMariella De Biasi
Dec 17, 2014·Autophagy·Natalie SpangChristian Behl
Aug 19, 2016·Frontiers in Oncology·Pratikkumar Harsukhbhai VekariaRekha Rao
Feb 20, 2015·Molecular Biology of the Cell·Jessica MerullaMaurizio Molinari
Oct 24, 2018·The Journal of Clinical Investigation·Sungro JoAntonio C Bianco
Mar 5, 2019·Cellular and Molecular Life Sciences : CMLS·Selma Yilmaz Dejgaard, John F Presley
Nov 1, 2016·The EMBO Journal·Chrisovalantis PapadopoulosHemmo Meyer
Jun 28, 2019·Frontiers in Neuroscience·Thomas J HedlAlbert Lee
Jul 1, 2017·Frontiers in Molecular Biosciences·Yihong YeDi Xia
Aug 19, 2018·Scientific Reports·Ana C BentoAlbert Neutzner
May 23, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Harshada KetkarPenghua Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.