Protein isolation from blue mussels (Mytilus edulis) using an acid and alkaline solubilisation technique--process characteristics and functionality of the isolates

Journal of the Science of Food and Agriculture
Patroklos Vareltzis, Ingrid Undeland

Abstract

The pH shift method was developed to isolate proteins from low-value raw materials by solubilisation at high or low pH followed by precipitation. In this paper the application of the pH shift method on isolated mussel (Mytilus edulis) meat and whole mussels is reported. Highest protein solubilisation was achieved at pH values of 2.6 and 12. The optimum precipitation pH values were established as around 5.8 following acid solubilisation and 5.2 following alkaline solubilisation. Protein recoveries were 430 and 580 g kg(-1) with the acid and alkaline processes respectively. Using whole crushed mussels, the corresponding recoveries were 310 and 480 g kg(-1). Process modifications to further improve protein recovery resulted in only a marginal increase. Lipid oxidation was not induced during pH shift processing, but heavy proteolysis occurred during the acid process version. Proteolysis could not be prevented by porcine plasma protein. Alkali-produced proteins performed better in all functionality tests compared with acid-produced proteins. The acid process removed slightly more lipids, recovered relatively more cysteine, methionine and lysine and resulted in whiter isolates. The pH shift method can be successfully used to extract ...Continue Reading

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Oct 31, 2007·Journal of Agricultural and Food Chemistry·Patroklos Vareltzis, Herbert O Hultin
Sep 11, 2010·Journal of Agricultural and Food Chemistry·Sofia K Marmon, Ingrid Undeland

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Citations

Nov 19, 2013·Archives of Gerontology and Geriatrics·Yue ZhouBei-Wei Zhu
Jul 23, 2019·Journal of the Science of Food and Agriculture·Vijay Kumar Reddy SurasaniI P Lakshmisha
Oct 20, 2018·Journal of Food Science and Technology·Xiangming QiXiangzhao Mao
Sep 16, 2017·Journal of the Science of Food and Agriculture·Sarah A LynchAnne Maria Mullen
Sep 12, 2018·Environmental Science and Pollution Research International·Vijay Kumar Reddy SurasaniAbhed Pandey
Dec 20, 2020·Comprehensive Reviews in Food Science and Food Safety·Xue ZhaoGuanghong Zhou
Mar 7, 2021·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Luis U Medina UzcáteguiGabriela Martínez Bordes
Jun 28, 2018·Meat Science·Sarah A LynchCarlos Álvarez

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