PMID: 9166715Jun 1, 1997Paper

Protein kinase A coordinately regulates both basal expression and cyclic AMP-mediated induction of three catecholamine-synthesizing enzyme genes

Journal of Neurochemistry
O HwangK S Kim

Abstract

Studies have shown that the cyclic AMP-regulated pathway is involved in the activation of tyrosine hydroxylase (TH) and in the induction of gene expression of the three catecholamine-synthesizing enzymes, TH, dopamine beta-hydroxylase (DBH), and phenylethanolamine N-methyltransferase (PNMT). In the present study we investigated further the role of protein kinase A (PKA) in the regulation of both basal and cyclic AMP-inducible transcription of the three catecholamine-synthesizing enzymes in primary cultured bovine chromaffin cells by using the PKA-specific inhibitor N-[2-(p-bromocinnamylamine)ethyl]-5-isoquinolinesulfonamide (H-89). In the presence of 40 microM H-89, mRNA levels of TH, DBH, and PNMT were reduced to 17 +/- 8, 19 +/- 8, and 14 +/- 2% of the untreated control, respectively, in 24 h, and intracellular norepinephrine and epinephrine levels were decreased to 20 and 34%, respectively, in 72 h. At 20 microM, although the basal enzyme gene expression levels were little affected, their induction by forskolin was abolished and norepinephrine and epinephrine levels fell to 55 and 74%. This reduction in catecholamines at 20 microM was probably due to changes in the phosphorylation state of TH, as its enzymatic activity was f...Continue Reading

Citations

Jul 13, 2004·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Xuan WangXiaomin Wang
Jan 15, 2003·Journal of Neuroscience Research·Carmen MontielAna M Cárdenas
Apr 29, 2006·Cellular and Molecular Neurobiology·Dona Lee Wong
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Nathalie PizzinatAngelo Parini
Mar 20, 2003·Journal of Neuroscience Research·Donghou KimOnyou Hwang
Mar 20, 2002·Molecular Pharmacology·Seong Who KimOnyou Hwang
May 13, 1999·Brain Research. Developmental Brain Research·F B Pliego RiveroH F Bradford
May 29, 2002·Brain Research Bulletin·E Arenas
Jan 31, 2007·Cellular Signalling·Larisa BobrovskayaPeter R Dunkley

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