Protein kinase A signalling in Schistosoma mansoni cercariae and schistosomules

International Journal for Parasitology
Natasha L HirstAnthony J Walker

Abstract

Cyclic AMP (cAMP)-dependent protein kinase/protein kinase A regulates multiple processes in eukaryotes by phosphorylating diverse cellular substrates, including metabolic and signalling enzymes, ion channels and transcription factors. Here we provide insight into protein kinase A signalling in cercariae and 24h in vitro cultured somules of the blood parasite, Schistosoma mansoni, which causes human intestinal schistosomiasis. Functional mapping of activated protein kinase A using anti-phospho protein kinase A antibodies and confocal laser scanning microscopy revealed activated protein kinase A in the central and peripheral nervous system, oral-tip sensory papillae, oesophagus and excretory system of intact cercariae. Cultured 24h somules, which biologically represent the skin-resident stage of the parasite, exhibited similar activation patterns in oesophageal and nerve tissues but also displayed striking activation at the tegument and activation in a region resembling the germinal 'stem' cell cluster. The adenylyl cyclase activator, forskolin, stimulated somule protein kinase A activation and produced a hyperkinesia phenotype. The biogenic amines, serotonin and dopamine known to be present in skin also induced protein kinase A ...Continue Reading

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J FuhlendorffT W Schwartz
Dec 1, 1974·Experimental Parasitology·F J Ramalho-PintoJ Pellegrino
Mar 6, 1998·Molecular and Cellular Biology·R D CauthronR A Steinberg
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·X ChengS S Taylor
Dec 26, 2001·Chemical Reviews·J B Shabb
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrzej SlominskiOlle Johansson
Feb 15, 2003·Trends in Parasitology·Rachel S Curwen, R Alan Wilson
Jan 13, 2004·Physiological Reviews·Kjetil Taskén, Einar Martin Aandahl
Apr 24, 2004·Trends in Parasitology·Adrian P Mountford, Francois Trottein
Jul 2, 2004·The Journal of Biological Chemistry·Judith E HumphriesTim A Day
Nov 5, 2004·American Journal of Human Genetics·Lauren A WeissCarole Ober
Jun 28, 2005·Nucleic Acids Research·Hsien-Da HuangJorng-Tzong Horng
May 16, 2006·Nature Cell Biology·Madhusudan NatarajanRama Ranganathan
Nov 13, 2007·Cellular Signalling·William A Sands, Timothy M Palmer
Dec 25, 2007·Parasitology Research·Simone Haeberlein, Wilfried Haas
Jul 1, 2008·Experimental Parasitology·Wilfried HaasEveline Zoppelli
Jul 17, 2008·PLoS Neglected Tropical Diseases·Elizabeth HansellJames H McKerrow
Aug 13, 2008·Infection and Immunity·Yi BaoHuan Huang
Apr 14, 2009·International Journal for Parasitology·Paul McVeighAaron G Maule
Jun 17, 2009·Protein Science : a Publication of the Protein Society·Robert A RomanoSusan S Taylor
Jun 24, 2009·Molecular and Biochemical Parasitology·Amira Taman, Paula Ribeiro
Aug 27, 2009·PLoS Neglected Tropical Diseases·Brett E Swierczewski, Stephen J Davies
Jan 26, 2010·International Journal for Parasitology·Brett E Swierczewski, Stephen J Davies
Jan 30, 2010·Experimental Parasitology·Brett E Swierczewski, Stephen J Davies
Feb 13, 2010·Journal of Molecular Endocrinology·Guillaume Pidoux, Kjetil Taskén
Nov 4, 2010·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Jan 29, 2011·BMC Cell Biology·Margarida RessurreiçãoAnthony J Walker
May 10, 2011·BMC Genomics·Luiza F AndradeGuilherme Oliveira
Jul 27, 2011·Molecular and Biochemical Parasitology·Margarida RessurreiçãoAnthony J Walker
Aug 31, 2011·Journal of Visualized Experiments : JoVE·John N Milligan, Emmitt R Jolly
Oct 22, 2011·Parasites & Vectors·Anthony John Walker
Nov 26, 2011·Nucleic Acids Research·Flora J Logan-KlumplerMatthew Berriman
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Malik M KeshwaniSusan S Taylor
Apr 25, 2012·Invertebrate Neuroscience : in·Paula RibeiroNelly El-Sakkary
Oct 4, 2012·Biochimica Et Biophysica Acta·Derek A Mann, Fiona Oakley
Dec 1, 2012·The Journal of Experimental Biology·Felix TiburcyHelmut Wieczorek

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Citations

Jan 9, 2018·The Journal of Infectious Diseases·Maxine McKenzieAnthony J Walker
Jan 23, 2020·Expert Review of Proteomics·Naina AroraAmit Prasad
Feb 10, 2018·PLoS Neglected Tropical Diseases·Federico CamiciaMara C Rosenzvit

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