Protein kinase C activation by serotonin potentiates agonist-induced stimulation of cAMP production in cultured rat retinal pigment epithelial cells

Experimental Eye Research
M NashN N Osborne

Abstract

Serotonin stimulates inositol phosphate production and intracellular calcium mobilization in cultured rat retinal pigment epithelial (RPE) cells through interaction with 5-HT2A receptors, but decreases cAMP production in cultured human RPE cells via 5-HT1A receptors. Studies were therefore undertaken to investigate the effect of serotonin on the cAMP system in rat RPE cells. Exposure of cultured rat RPE cells to serotonin (100 microM) for 10 minutes had no effect on the basal levels of cAMP. However, a 5 minute preincubation with serotonin potentiated the production of cAMP induced by a 5 minute exposure to forskolin (5 microM), isoproterenol (1 microM) and 5'-[N-ethylcarboxamido]-adenosine (10 microM) by 133.0%, 296.8% and 651.9%, respectively. This effect of serotonin was dose-dependent on forskolin and 5'-[N-ethylcarboxamido]-adenosine with half-maximal effects close to those reported for its action on inositol phosphates production. The antagonists ketanserin, methysergide and spiperone attenuated the action of serotonin, while yohimbine and spiroxatrine were ineffectual, thus indicating that the potentiating effect was through the 5-HT1A receptor. Incubation of cultured rat RPE cells with bradykinin stimulates inositol pho...Continue Reading

Citations

Jun 4, 2010·Antioxidants & Redox Signaling·Michael V Cohen, James M Downey
Jul 29, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Atsushi KunoJames M Downey
Feb 6, 2010·Physiology·Brian M Kevany, Krzysztof Palczewski
Oct 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·Samarjit BhattacharyyaMitradas M Panicker
Nov 26, 2010·Biochimica Et Biophysica Acta·John P HeadrickLuke J Haseler
Dec 3, 2014·Biochemical Pharmacology·Aiysha Thompson, Venkateswarlu Kanamarlapudi
Mar 23, 2005·Experimental Eye Research·David J CollisonJulie Sanderson
Jul 1, 2005·Physiological Reviews·Olaf Strauss
Mar 21, 2021·Ophthalmology and Therapy·Hedayat JavidiSajjad Mahmood

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