PMID: 7520282Aug 11, 1994Paper

Protein kinase C activation inhibits cytokine-induced nitric oxide synthesis in vascular smooth muscle cells

Biochimica Et Biophysica Acta
Y J GengG K Hansson

Abstract

Vascular smooth muscle cells (SMC) respond by relaxation to nitric oxide (NO) released from the endothelium which expresses a constitutive, Ca(2+)-dependent NO synthase (cNOS). SMC can, however, produce NO themselves upon stimulation by proinflammatory cytokines which induce expression of an inducible, Ca(2+)-independent NO synthase (iNOS). Protein kinase C represents another important second messenger system involved in the regulation of SMC contraction. We have investigated iNOS expression and NO synthesis in rat vascular SMC treated with the cytokines, IFN gamma and TNF alpha, in the presence or absence of the activator of protein kinase C, beta-phorbol-12-myristate 13-acetate (PMA). Treatment with PMA did not induce any significant accumulation of nitrite, a major stable metabolite of NO, in SMC. When added simultaneously with the cytokines, PMA significantly reduced nitrite accumulation induced by cytokine stimulation in a dose-dependent fashion. This inhibitory effect was mediated by activation of PKC since calphostin C, a specific PKC inhibitor, abolished the PMA effect. Further analysis of iNOS mRNA with a rat iNOS cDNA probe demonstrated that addition of PMA reduced expression of SMC iNOS mRNA, indicating that the anta...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S LamasT Michel
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C J LowensteinS H Snyder
Sep 4, 1992·Cell·C J Lowenstein, S H Snyder
Nov 1, 1992·Hypertension·J E Andrea, M P Walsh
Nov 16, 1992·Biochemical and Biophysical Research Communications·A SevernF Y Liew
Jan 1, 1991·Pharmacology & Therapeutics·S Stabel, P J Parker
Mar 15, 1989·Biochemical and Biophysical Research Communications·E KobayashiT Tamaoki
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L J IgnarroG Chaudhuri
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
Feb 26, 1993·Biochemical and Biophysical Research Communications·Y NunokawaS Tanaka
Jun 15, 1993·Biochemical and Biophysical Research Communications·H TsukaharaM S Goligorsky

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Citations

May 1, 1997·Disease-a-month : DM·R G KilbournC Szabó
May 30, 1998·British Journal of Pharmacology·H KleinertU Förstermann
Aug 1, 1995·Alcoholism, Clinical and Experimental Research·W E Lands
Mar 1, 1996·Journal of Pineal Research·G J Maestroni
Aug 1, 1997·International Journal of Cardiology·R W Wissler, P D Group
Apr 25, 2006·European Journal of Pharmacology·María E PachecoMercedes Salaices
Dec 22, 1999·The American Journal of Physiology·M Liang, F G Knox
Jan 25, 2000·American Journal of Physiology. Endocrinology and Metabolism·M B Ganz, A Seftel

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