Protein kinase C activation of a blebbistatin sensitive contractile component in the wall of hypertrophying mouse urinary bladder

Neurourology and Urodynamics
Lena BobergAnders Arner

Abstract

To examine the role of protein kinase C (PKC) and non-muscle myosin in regulation of wall tension in the hypertrophied urinary bladder. A partial urinary outflow obstruction was induced in the mouse. Tissue strips from sham operated controls and obstructed bladders were examined in vitro with quantitative gel electrophoresis, immunohistochemistry, and in vitro force recordings. Outlet obstruction (14-18 days) induced a significant growth of the bladder, 73 ± 6.13 mg compared to 19 ± 1 13 mg in sham operated controls. The hypertrophying bladder tissue had increased expression of non-muscle myosin B (SMemb) mainly localized to serosa and suburothelium. Direct activation of PKC with PDBu did not alter force in the control urinary bladder. In contrast, PDBu initiated a prominent and sustained contraction which had an increased sensitivity to the myosin type II inhibitor blebbistatin. PKC activates a significant contractile response in the wall of the hypertrophying urinary bladder, possibly supported by non-muscle myosin. This contractile component is not contributing to the physiological response to muscarinic stimulation, but might be separately regulated by other, yet unknown, mechanisms.

References

May 1, 1991·The American Journal of Physiology·U MalmqvistB Uvelius
Apr 1, 1995·Journal of Applied Physiology·M RoelofsR M Levin
Jul 3, 1998·Naunyn-Schmiedeberg's Archives of Pharmacology·M J LinS Y Lin-Shiau
Jun 15, 2000·Nature Cell Biology·I MoranoM Bader
Mar 15, 2003·Science·Aaron F StraightTimothy J Mitchison
Apr 2, 2003·The Journal of General Physiology·Mia LöfgrenAnders Arner
Dec 10, 2003·Urology·Karl Erik Andersson
Apr 22, 2004·Advances in Experimental Medicine and Biology·Valéria Lamounier-ZepterIngo Morano
Nov 19, 2004·Journal of Muscle Research and Cell Motility·John LimouzeJames R Sellers
May 11, 2006·Pflügers Archiv : European journal of physiology·Albert Y RheeFrank V Brozovich
Nov 30, 2006·The Journal of Pharmacology and Experimental Therapeutics·Thomas J EddingerPaul H Ratz
Jun 20, 2008·American Journal of Physiology. Cell Physiology·R Sjuve ScottA Arner
Apr 22, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shaohua ChangSamuel Chacko

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Citations

Mar 11, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Andrzej WróbelEwa Poleszak

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