Protein kinase C-alpha attenuates cholinergically stimulated gastric acid secretion of rabbit parietal cells

British Journal of Pharmacology
Michael FährmannUrsula Seidler

Abstract

(1) The phorbolester 12-O-tetradecanoyl phorbol-13-acetate (TPA), an activator of protein kinase C (PKC), inhibits cholinergic stimulation of gastric acid secretion. We observed that this effect strongly correlated with the inhibition of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) activity in rabbit parietal cells. (2) The aim of this study was to specify the function of PKC-alpha in cholinergically stimulated H(+) secretion. PKC-alpha represents the only calcium-dependent PKC isoenzyme that has been detected in rabbit parietal cells. (3) Gö 6976, an inhibitor of calcium-dependent PKC, concentration-dependently antagonized the inhibitory effect of TPA, and, therefore, revealed the action of PKC-alpha on carbachol-induced acid secretion in rabbit parietal cells. (4) TPA exerted no additive inhibition of carbachol-stimulated acid secretion if acid secretion was partially inhibited by the potent CaMKII inhibitor 1-[N,O-bis(5-isoquinolinsulfonyl)-N-methyl-L-tyrosyl]-4-phenyl-piperazine (KN-62). (5) Since both kinase modulators, TPA and KN-62, affected no divergent signal transduction pathways in the parietal cell, an in vitro model has been used to study if PKC directly targets CaMKII. CaMKII purified from parietal cell-...Continue Reading

References

Sep 1, 1987·Canadian Journal of Physiology and Pharmacology·M R Brown, C S Chew
Dec 16, 1987·Biochemical and Biophysical Research Communications·W BeilK F Sewing
Jan 1, 1995·Annual Review of Physiology·A P Braun, H Schulman
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·R P XiaoE G Lakatta
Mar 15, 1993·Biochemical and Biophysical Research Communications·G Y ZhangR K Sharma
Apr 1, 1997·Current Opinion in Cell Biology·A C Newton
Oct 6, 1997·The Journal of Membrane Biology·T Urushidani, J G Forte
Jan 15, 1999·Clinical and Experimental Pharmacology & Physiology·C A Kanashiro, R A Khalil
Jul 31, 1999·The Journal of Biological Chemistry·L BrockeH Schulman
Nov 30, 2000·The Journal of Biological Chemistry·T R SoderlingD Brickey
Jul 12, 2002·British Journal of Pharmacology·Michael FährmannUrsula Seidler
Jul 9, 2003·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·K LiuD Xue
Oct 13, 2006·Current Opinion in Gastroenterology·Mitchell L Schubert

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Citations

Jan 13, 2006·Biochimica Et Biophysica Acta·Michael Fährmann, Marc-André Kaufhold
Jan 11, 2013·Physiological Reviews·Sascha Kopic, John P Geibel

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