Protein kinase C and simulated ischemia possible aberrations of signal transduction during ischemia

Journal of Anesthesia
K IritaJ Yoshitake

Abstract

ATP depletion is always associated with prolonged ischemia. It was found that ATP affected calcium- and phospholipid-dependent activation of protein kinase C without hydrolysis of the nucleotide when the activation was monitored by an assay for [3H] 4-beta-phorbol-12, 13-dibutyrate binding activity in a reconstitution system having physiological concentrations of free calcium. When the ATP level was low, an increase in the free calcium concentration could not activate the enzyme. A decrease in pH exacerbated the depressed activation. The concentration of magnesium also affected the activation. On the other hand, free fatty acids, which increase during ischemia, were able to activate the enzyme at a low concentration of ATP in the absence of phorbol ester and phosphatidylserine. These results suggest that calcium- and phospholipid-dependent activation of protein kinase C is suppressed during ischemia, and that fatty acids in turn activate the enzyme. It is possible that ischemia interferes with normal signal transduction via the protein kinase C pathway and causes unusual protein phosphorylation.

References

Jan 17, 1986·Science·P W Hochachka
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·F VaccarinoE Costa
Jan 1, 1985·Progress in Brain Research·J H ChinR J DeLorenzo
Jun 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D UematsuA Karp
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B R GanongR M Bell
Jun 26, 1986·The New England Journal of Medicine·J Y CheungA Leaf
Jun 15, 1987·Biochemical and Biophysical Research Communications·K SekiguchiY Nishizuka
Jan 1, 1983·Annals of Neurology·M E Raichle
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R S TurnerJ F Kuo
Aug 1, 1984·Kidney International·E MatthysM Venkatachalam

❮ Previous
Next ❯

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.