Abstract
It has previously been demonstrated that microtubule-associated protein 2 (MAP2) is a good substrate for the purified protein kinase C [Tsuyama, S., Bramblett, G. T., Huang, K.-P. & Flavin, M. (1986) J. Biol. Chem. 261, 4110-4116; Akiyama, T., Nishida, E., Ishida, J., Saji, N., Ogawara, H., Hoshi, M., Miyata, Y. & Sakai, H. (1986) J. Biol. Chem. 261, 15648-15651]. We have shown here that phosphorylation of MAP2, catalyzed by protein kinase C, reduces the ability to induce tubulin polymerization. MAP2 is divided into two domains by digestion with alpha-chymotrypsin; the microtubule-binding and the non-binding (projection) domains. The limited chymotryptic digestion following phosphorylation of MAP2 by protein kinase C has shown that both the domains of MAP2 were phosphorylated by protein kinase C, 50-60% of the incorporated phosphates being detected in the microtubule-binding domain. Polypeptide fragments, containing the microtubule-binding domain of MAP2, were purified by DEAE-cellulose column chromatography after chymotryptic digestion of MAP2. The purified microtubule-binding fragments were competent to polymerize tubulin, and served as good substrates for protein kinase C. The phosphorylation of the microtubule-binding fragm...Continue Reading
References
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R D SlobodaP Greengard
Feb 1, 1979·The Journal of Cell Biology·H KimJ L Rosenbaum
Sep 1, 1978·The Journal of Cell Biology·L M Griffith, T D Pollard
Dec 1, 1978·European Journal of Biochemistry·W Herzog, K Weber
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M D WeingartenM W Kirschner
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D B Murphy, G G Borisy
Sep 1, 1985·Journal of Neurochemistry·J R GoldenringR J DeLorenzo
Jan 1, 1987·Journal of Neurochemistry·M A HernándezJ Avila
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M L VallanoR J DeLorenzo
Mar 1, 1985·Journal of Neurochemistry·H YamamotoE Miyamoto
Aug 15, 1970·Nature·U K Laemmli
Dec 1, 1983·European Journal of Biochemistry·A S Murthy, M Flavin
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R Vallee
Sep 1, 1981·The Journal of Cell Biology·R B ValleeW E Theurkauf
Oct 23, 1984·Biochemistry·E NishidaH Sakai
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R B Vallee, S E Davis
Jun 15, 1984·European Journal of Biochemistry·R G Burns, K Islam
May 1, 1980·The Journal of Cell Biology·S D MacLean-Fletcher, T D Pollard
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L Jameson, M Caplow
Dec 15, 1982·Biochemical and Biophysical Research Communications·T Yamauchi, H Fujisawa
Jan 26, 1981·FEBS Letters·K Islam, R Burns
Aug 1, 1981·Journal of Biochemistry·E NishidaH Sakai
May 27, 1980·Biochemistry·L JamesonM Caplow
Citations
Apr 1, 1991·Journal of Protein Chemistry·S D YangY G Lai
Aug 1, 1993·Journal of Protein Chemistry·S D YangW H Chan
Nov 1, 1992·The Histochemical Journal·B M Riederer
Nov 1, 1990·Brain Research. Developmental Brain Research·B M RiedererG M Innocenti
Dec 21, 1995·Brain Research. Developmental Brain Research·S M Dudek, G V Johnson
Jul 1, 1995·Brain Research. Molecular Brain Research·H M Johnston, B J Morris
Dec 1, 1992·Neurochemistry International·C Tanaka, N Saito
Jan 15, 1996·Molecular and Cellular Endocrinology·J P Liu
Jan 1, 1990·Neuroscience·P Friedrich
Jun 1, 1993·Neuroscience·R J MontoroJ López-Barneo
Aug 8, 1997·Brain Research·C SánchezJ Avila
Aug 27, 1997·European Journal of Pharmacology·S MiyamotoA Aoba
Feb 14, 2002·Mechanisms of Ageing and Development·Maurizio SabbatiniRoberto Avola
May 22, 2002·Neurochemistry International·Regina CorbalánVicente Felipo
Mar 8, 2000·Progress in Neurobiology·C SánchezJ Avila
Dec 18, 2001·Neuroscience·C SánchezF J Díez-Guerra
Jun 9, 1998·Cellular Signalling·C Keenan, D Kelleher
Apr 12, 2001·The European Journal of Neuroscience·M LlansolaV Felipo
Sep 22, 1999·European Journal of Biochemistry·T SapirO Reiner
Dec 18, 2002·Journal of Neurochemistry·C Abi FarahN Leclerc
Oct 12, 2000·Molecular Biology of the Cell·R S Ozer, S Halpain
Aug 11, 1999·Human Molecular Genetics·D HoreshO Reiner
Jan 15, 1992·European Journal of Biochemistry·M HoshiE Nishida
Nov 1, 1996·European Journal of Biochemistry·C SánchezJ Avila
Jan 1, 1992·The European Journal of Neuroscience·Beat M. Riederer, Giorgio M. Innocenti
Jul 1, 1993·The European Journal of Neuroscience·J Díaz-NidoJ Avila
Jan 1, 1990·Journal of Neurochemistry·J Díaz-NidoJ Avila
Mar 15, 2005·BMC Neuroscience·Cindy K Reiter-Funk, Douglas P Dohrman
Mar 2, 2010·Cell Structure and Function·Koji Ikegami, Mitsutoshi Setou
Oct 1, 1991·The European Journal of Neuroscience·Beat M. Riederer, Giorgio M. Innocenti
Jun 25, 2015·IUBMB Life·Renu Mohan, Annie John
Nov 21, 2000·Molecular and Cellular Neurosciences·H A Burgess, O Reiner
Jan 27, 2004·Neuron·Teruyuki TanakaJoseph G Gleeson
Feb 1, 1996·Neuron·E M Quinlan, S Halpain
Jun 4, 1990·Brain Research·A KoseC Tanaka
Oct 6, 2016·Critical Reviews in Toxicology·Mohamed B Abou-DoniaAshly Sobel Sokol
Sep 25, 1990·Biochemistry·J C Joly, D L Purich
Sep 24, 1991·Biochemistry·A MoriH Sakai
Jun 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A MatsunoK Watanabe
Oct 27, 2017·Toxicology and Industrial Health·Mohamed Bahie Abou-DoniaLuke Curtis
Jul 11, 1998·Stroke; a Journal of Cerebral Circulation·F E SieberL J Martin
Oct 26, 2014·Molecular Neurobiology·Ana Gabriela HenriquesOdete A B da Cruz E Silva
Nov 1, 1995·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Jonathan D Lane, Howard Stebbings
Nov 1, 1993·Journal of Neurobiology·C M Beaman-Hall, M L Vallano
Mar 5, 2017·The Journal of Biological Chemistry·Séverine JansenLukáš Žídek
Apr 6, 2013·Journal of Toxicology and Environmental Health. Part a·Mohamed B Abou-DoniaMichel F A Mulder
Aug 1, 1996·Neurosurgery·D YoshidaA Teramoto
Jan 28, 2021·Brain Sciences·Mohamed B Abou-DoniaKimberly Sullivan
Sep 27, 1990·Biochimica Et Biophysica Acta·A Rivas-BerríosR B Maccioni
Feb 1, 1991·Mechanisms of Development·A FellousC Jessus