Protein kinase C-delta regulates HIV-1 replication at an early post-entry step in macrophages.

Retrovirology
Xavier ContrerasElmostafa Bahraoui

Abstract

Macrophages, which are CD4 and CCR5 positive, can sustain HIV-1 replication for long periods of time. Thus, these cells play critical roles in the transmission, dissemination and persistence of viral infection. Of note, current antiviral therapies do not target macrophages efficiently. Previously, it was demonstrated that interactions between CCR5 and gp120 stimulate PKC. However, the PKC isozymes involved were not identified. In this study, we identified PKC-delta as a major cellular cofactor for HIV-1 replication in macrophages. Indeed, PKC-delta was stimulated following the interaction between the virus and its target cell. Moreover, inhibition of PKC-delta blocked the replication of R5-tropic viruses in primary human macrophages. However, this inhibition did not have significant effects on receptor and co-receptor expression or fusion. Additionally, it did not affect the formation of the early reverse transcription product containing R/U5 sequences, but did inhibit the synthesis of subsequent cDNAs. Importantly, the inhibition of PKC-delta altered the redistribution of actin, a cellular cofactor whose requirement for the completion of reverse transcription was previously established. It also prevented the association of the...Continue Reading

References

Mar 1, 1992·European Journal of Biochemistry·U SchubertT Porstmann
Jun 1, 1990·Virology·B GuyM P Kieny
Aug 18, 1988·Nature·W W LamphI M Verma
Mar 11, 1995·Nucleic Acids Research·M HottigerU Hübscher
Jun 20, 1997·Science·J M OrensteinS M Wahl
Dec 31, 1997·The Journal of Experimental Medicine·C B DavisD R Littman
Oct 29, 1998·The Journal of Biological Chemistry·X Yang, D Gabuzda
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·C VitaJ C Gluckman
Mar 30, 2001·The Journal of Biological Chemistry·G HassaïneE Decroly
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yamina Bennasser, Elmostafa Bahraoui
Apr 14, 2004·The Journal of Experimental Medicine·Qinxue HuRobin J Shattock
Jul 31, 2004·The Journal of Biological Chemistry·Samuel A WilliamsWarner C Greene
Sep 9, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Fanny TurlureAlan Engelman
Dec 14, 2004·American Journal of Physiology. Cell Physiology·Nicole D SmallwoodCarole M Liedtke
Sep 23, 2006·AIDS Research and Human Retroviruses·David WarrilowDavid Harrich
Sep 23, 2006·Nature Methods·Nathalie ArhelPierre Charneau
Apr 4, 2007·The Journal of Biological Chemistry·Maria L TorgersenKirsten Sandvig
Jul 31, 2007·Journal of Cancer Research and Clinical Oncology·D BreitkreutzT Tennenbaum
May 5, 2009·Cell·Kosuke MiyauchiGregory B Melikyan
Dec 31, 2009·PLoS Pathogens·Yuntao Wu, Alyson Yoder

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Citations

Apr 2, 2013·Lancet·Christine KatlamaRafick P Sekaly
Oct 6, 2012·Retrovirology·Herwig KoppensteinerMichael Schindler
Oct 8, 2013·Journal of Molecular Biology·Sarah S WilsonJason G Smith
Sep 26, 2015·Clinical Epigenetics·Amit KumarGeorges Herbein
Jul 12, 2014·Virology·Diego Pandeló JoséRenato Santana Aguiar
Nov 19, 2013·Cell Host & Microbe·Yosef SaboMojgan H Naghavi
Oct 8, 2014·AIDS Research and Human Retroviruses·Guochun Jiang, Satya Dandekar
Mar 27, 2015·PLoS Pathogens·David A GalbraithChristina Grozinger
May 19, 2017·FEMS Microbiology Reviews·María Eugenia Pachón-IbáñezJavier Sánchez-Céspedes
Jan 26, 2018·PloS One·Hsing-I HuangJhao-Yin Lin
May 10, 2019·Antiviral Research·Zoé LamaCécile Lagaudrière-Gesbert
May 3, 2021·Microbial Pathogenesis·Subhash Singh SolankiSyed Azmal Ali
Aug 26, 2021·Retrovirology·E A Nickoloff-BybelP J Gaskill

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Methods Mentioned

BETA
PMA
ELISA
antisense oligonucleotides
flow cytometry
FACS
PCR
confocal microscopy
transfection
FCS
antisense

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