Protein kinase C inhibition abrogates hepatic ischemic preconditioning responses

The Journal of Surgical Research
R RicciardiR S Chari

Abstract

A transient period of warm ischemia prior to a longer ischemic episode (ischemic preconditioning) protects the hepatic graft from cold ischemia. The mechanism for this protection is unknown, as is the role of protein kinase C in ischemic preconditioning responses. Livers from 40 kg Yorkshire pigs were harvested and subjected to 2 h of cold ischemia (n = 6) (control). Another group of harvested livers was pretreated with a 15-min ischemic period followed by 15 min of in situ perfusion with (n = 5) or without (n = 5) a protein kinase C inhibitor, chelerythrine. Following cold ischemia, all grafts were reperfused on a perfusion circuit and the following variables evaluated: (1) hepatic graft function, (2) graft circulatory impairment, (3) hepatocellular damage, and (4) endothelial cell damage. Protein kinase C levels were also evaluated by Western blot in the cytoplasm of all grafts. Ischemic preconditioned grafts demonstrate improved graft function, reduced graft circulatory impairment, and reduced endothelial cell damage as compared to cold ischemia controls. When preconditioned grafts were pretreated with chelerythrine, graft function, graft circulatory impairment, and endothelial cell damage were no different than cold ischemi...Continue Reading

References

Jan 1, 1995·Circulation Research·M B MitchellA Banerjee
Apr 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C PeraltaJ Roselló-Catafau
Dec 2, 1998·Basic Research in Cardiology·R BolliJ Zhang
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C PeraltaJ Roselló-Catafau

❮ Previous
Next ❯

Citations

May 1, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Eduardo E Montalvo-JaveDavid A Geller
Jul 8, 2011·World Journal of Gastroenterology : WJG·Hassen Ben AbdennebiJoan Roselló-Catafau
Apr 5, 2013·World Journal of Gastroenterology : WJG·Gourab DattaBrian R Davidson
Feb 13, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Joan Torras AmbrosJosep M Grinyo Boira
Apr 26, 2008·Transplant International : Official Journal of the European Society for Organ Transplantation·Leonardo BaiocchiMario Angelico
Apr 12, 2005·The Journal of Surgical Research·Jun W UmEdward C Mun
Apr 27, 2005·The British Journal of Surgery·N R BangaK R Prasad
Jun 24, 2010·Transplantation·Hjalmar R BoumaRobert H Henning
Nov 2, 2005·The Journal of Pathology·R Franco-GouC Peralta
Mar 24, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Matteo CesconAntonio Daniele Pinna
Jan 27, 2006·World Journal of Gastroenterology : WJG·Ming-Qing XuLu-Nan Yan
Nov 5, 2003·Gastroenterology·Rita Carini, Emanuele Albano
Sep 14, 2004·Gastroenterology·Rita CariniEmanuele Albano
Dec 29, 2006·Cirugía española·Luis GrandeCarmen Peralta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
F Javier García-CriadoJosé M López-Novoa
Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
Matteo CesconAntonio Daniele Pinna
© 2021 Meta ULC. All rights reserved