Abstract
Previous investigations have suggested that protein kinase C (PKC) may regulate guinea pig eosinophil responses through a suppressive "negative feedback" mechanism. Using the selective PKC inhibitors bisindolylmaleimide I (Bis I, GF 109203X) and calphostin C, we examined the role of PKC in platelet-activating factor (PAF)-induced respiratory burst and generation of arachidonic acid metabolites in human peripheral blood eosinophils. Bis I inhibited PAF-induced generation of superoxide anion with substantially lower potency (geometric mean IC50 = 1.41 microM, 95% CI 0.94-2.11 microM) than it exhibited against responses to the phorbol esters 4-beta-phorbol 12-myristate 13-acetate (PMA; IC50 = 0.25 microM, 0.09-0.72 microM; P < 0.01) and 4-beta-phorbol 12,13-dibutyrate (IC50 = 0.48 microM, 0.20-1.14 microM; P < 0.05). The production of thromboxane (measured as TxB2) induced by 1 microM PAF was increased significantly by Bis I at concentrations of 1 microM (162 +/- 7.5% of control PAF response; P < 0.01) and 10 microM (194 +/- 17%; P < 0.001); TxB2 release induced by PMA was unaffected by concentrations of Bis I up to 1 microM and inhibited by 10 microM Bis I (48 +/- 11%; P < 0.05). Bis I (1 microM) significantly increased both thro...Continue Reading
References
Mar 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S D Shukla
Aug 6, 1992·European Journal of Pharmacology·S Müller, S Nigam
Dec 18, 1991·European Journal of Biochemistry·J Wijkander, R Sundler
Dec 15, 1991·Journal of Immunological Methods·T T HanselC Walker
Jan 1, 1992·Journal of Medicinal Chemistry·P D DavisS E Turner
Oct 1, 1990·Archives of Biochemistry and Biophysics·W ChaoM S Olson
Apr 1, 1990·American Journal of Respiratory Cell and Molecular Biology·T YukawaP J Barnes
Mar 15, 1989·Biochemical and Biophysical Research Communications·E KobayashiT Tamaoki
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M YamazakiR I Sha'afi
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P F WellerR A Lewis
Sep 15, 1995·The Biochemical Journal·R S PerkinsM A Giembycz
Nov 1, 1994·American Journal of Respiratory Cell and Molecular Biology·C KroegelP J Barnes
Dec 1, 1993·Journal of Leukocyte Biology·T van der BruggenL Koenderman
Aug 1, 1996·British Journal of Pharmacology·H TenorJ K Shute
Citations
Dec 19, 2001·Journal of Cellular Physiology·J L Bankers-FulbrightS M O'Grady
Jan 5, 2000·European Journal of Pharmacology·G P SamokhinR A Henriksen
Oct 3, 2003·Veterinary Immunology and Immunopathology·E C GreenawayN T Goode
Oct 19, 2001·British Journal of Pharmacology·O T LynchM A Lindsay
Jul 11, 2000·Immunology·G DentK F Rabe
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·D Schmidt, K F Rabe
Mar 6, 2004·American Journal of Respiratory and Critical Care Medicine·Gordon DentRatko Djukanovic
Aug 6, 2010·International Immunopharmacology·Anwar Matar HasanGordon Dent
Aug 7, 2014·Immunobiology·Gordon DentFiona M Leslie
Nov 1, 2006·Allergology International : Official Journal of the Japanese Society of Allergology·Takafumi YamaguchiMasahiko Kato
Sep 26, 2000·Pulmonary Pharmacology & Therapeutics·M A GiembyczM A Lindsay
Mar 25, 2006·International Archives of Allergy and Immunology·G Sandra TjabringaPieter S Hiemstra
May 3, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T OshiroK Shirato
Apr 13, 2000·FEBS Letters·N GeijsenP J Coffer
Aug 4, 2005·Allergy·R K MishraG Dent
Sep 16, 2005·Immunology·Masahiko KatoHirokazu Kimura
Aug 9, 2018·Scientific Reports·Carlos Fernando Odir Rodrigues MeloRodrigo Ramos Catharino
Aug 13, 1999·The American Journal of Physiology·M GekleW H Dantzler
Mar 24, 2000·Progress in Lipid Research·S Ishii, T Shimizu
Aug 10, 2002·The Journal of Allergy and Clinical Immunology·Takumi TakizawaAkihiro Morikawa
Apr 22, 2003·The Journal of Allergy and Clinical Immunology·Satoshi Miike, Hirohito Kita