Protein kinase C involvement in maintenance and modulation of noradrenaline release in the mouse brain cortex

British Journal of Pharmacology
G E SchroederH Majewski

Abstract

1. The role of protein kinase C in the modulation of noradrenaline release was investigated in mouse cortical slices which were pre-incubated with [3H]-noradrenaline. The aim was to investigate the hypothesis that protein kinase C is activated during high levels of transmitter release to maintain transmitter output. 2. The protein kinase C activators, phorbol myristate acetate (0.01-0.3 microM) and to a greater extent 4 beta-phorbol 12,13-dibutyrate (0.01-0.3 microM) significantly enhanced stimulation-induced noradrenaline release whereas 4 alpha-phorbol 12,13-dibutyrate (0.1 microM) which does not activate protein kinase C was without effect. The effect of the protein kinase C activator, phorbol myristate acetate, on noradrenaline release was attenuated by the protein kinase C inhibitor, polymyxin B (21 microM) which by itself inhibited stimulation-induced noradrenaline release. 3. Protein kinase C was down-regulated by 10 h exposure of the cortical slices to 4 beta-phorbol 12,13-dibutyrate (1 microM). In this case the facilitatory effect of 4 beta-phorbol 12,13-dibutyrate (0.1 microM) on noradrenaline release was abolished as was the inhibitory effect produced by polymyxin B. This indicates that polymyxin B was acting selecti...Continue Reading

References

Jan 1, 1991·Pharmacology & Therapeutics·S Stabel, P J Parker
Jan 1, 1989·Naunyn-Schmiedeberg's Archives of Pharmacology·I F Musgrave, H Majewski
Jul 1, 1989·Physiological Reviews·K StarkeH Kilbinger
Jun 1, 1989·Trends in Pharmacological Sciences·U T Rüegg, G M Burgess
Sep 1, 1987·British Journal of Pharmacology·C AllgaierR Jackisch
Oct 1, 1985·Naunyn-Schmiedeberg's Archives of Pharmacology·A R WakadeT D Wakade
Feb 1, 1987·Journal of Neurochemistry·R A NicholsP Greengard
Dec 1, 1986·The American Journal of Physiology·H Shuntoh, C Tanaka
May 1, 1965·The Journal of General Physiology·C M ARMSTRONG, L BINSTOCK

❮ Previous
Next ❯

Citations

Mar 5, 1999·Molecular Neurobiology·P F VaughanC Peers
Mar 10, 2000·European Journal of Pharmacology·J KameiH Hitosugi
Nov 21, 2002·Neurochemistry International·Aline Araujo dos SantosElizabeth Giestal de Araujo
Jul 22, 1998·Progress in Neurobiology·H Majewski, L Iannazzo
Aug 1, 1997·Clinical and Experimental Pharmacology & Physiology·H MajewskiI F Musgrave
Sep 20, 2008·Neurochemical Research·Aline Araujo dos SantosElizabeth Giestal de Araujo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.