Protein kinase C isotypes theta, delta and eta in human lymphocytes: differential responses to signalling through the T-cell receptor and phorbol esters

Immunology
C KeenanD Kelleher

Abstract

The repertoire of novel and atypical protein kinase C (PKC) isotypes present in human T cells and their subcellular localization have not been fully characterized. We detected calcium-independent PKC activity in whole cell fractions from unstimulated peripheral blood lymphocytes (PBL). Towards an understanding of the role of PKC isoforms in lymphocyte activation we have studied the expression of calcium-independent PKC isoforms theta, delta and eta in PBL. With isoformspecific antibodies we detected the presence of PKC theta and delta in whole cell fractions from unstimulated human PBL by Western blot analysis. In addition, immunocytochemical analysis confirmed the presence of the novel PKC isoform PKC eta in PBL. Using immunocytochemistry, PKC theta, delta and eta had distinct patterns of redistribution following activation by phorbol myristate acetate (PMA). However, signalling through the T-cell receptor (TCR) did not appear to induce such changes in isoenzyme redistribution. These findings indicate that activation of lymphocytes either through the TCR-CD3 complex or with PMA induces different signalling pathways with respect to calcium-independent isoenzymes. Signalling through receptors other than the CD3 complex may be in...Continue Reading

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Citations

Jun 9, 1998·Cellular Signalling·C Keenan, D Kelleher
Jan 19, 1999·Annual Review of Cell and Developmental Biology·P GunningE Hardeman
May 26, 2005·International Immunopharmacology·Ming-Sing SiDominic C Borie
Dec 12, 2001·The Journal of Surgical Research·H W ChenR C Yang
Jul 13, 2005·Biochemical and Biophysical Research Communications·Michael FreeleyAideen Long
Dec 8, 2004·Seminars in Immunology·Santos MañesCarlos Martínez-A
Feb 18, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lubna H AbdullahC William Davis
Oct 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gabriela GorelikBruce Richardson
Jun 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J P HattonD Schmitt
Oct 23, 1997·Biochimica Et Biophysica Acta·C KeenanD Kelleher

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