Protein kinase C isozyme-specific potentiation of expressed Ca v 2.3 currents by acetyl-beta-methylcholine and phorbol-12-myristate, 13-acetate.

Brain Research
S RajagopalG L Kamatchi

Abstract

Protein kinase C (PKC) is implicated in the potentiation of Ca v 2.3 currents by acetyl-beta-methylcholine (MCh), a muscarinic M1 receptor agonist or phorbol-12-myristate, 13-acetate (PMA). The PKC isozymes responsible for the action of MCh and PMA were investigated using translocation as a measure of activation and with isozyme-selective antagonists and siRNA. Ca v channels were expressed with alpha1 2.3, beta1b and alpha2delta subunits and muscarinic M1 receptors in the Xenopus oocytes and the expressed currents (I Ba) were studied using Ba2+ as the charge carrier. Translocation of PKC isozymes to the membrane studied by Western blot revealed that all eleven known PKC isozymes are present in the Xenopus oocytes. Exposure of the oocytes to MCh led to the translocation of PKC alpha whereas PMA activated PKC betaII and epsilon isozymes. The action of MCh was inhibited by Go 6976, an inhibitor of cPKC isozymes or PKC alpha siRNA. PMA-induced potentiation of Ca v 2.3 currents was inhibited by CG533 53, a PKC betaII antagonist, betaIIV5.3, a peptide translocation inhibitor of PKC betaII or PKC betaII siRNA. Similarly, epsilonV1.2, a peptide translocation inhibitor of PKC epsilon or PKC epsilon siRNA inhibited PMA action. The inhibi...Continue Reading

References

Apr 1, 1992·The Journal of Cell Biology·W C WetselY A Hannun
Jan 1, 1991·Methods in Cell Biology·G H Leno, R A Laskey
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S G Rane, K Dunlap
Jan 1, 1987·CRC Critical Reviews in Biochemistry·N Dascal
Jun 1, 1988·Trends in Neurosciences·T P Snutch
Jun 1, 1993·Pharmacology & Therapeutics·M P Caulfield
Feb 1, 1997·Pflügers Archiv : European journal of physiology·K E Pemberton, S V Jones
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·R D Zühlke, H Reuter
Apr 29, 1998·Trends in Neurosciences·D Walker, M De Waard
Apr 25, 2000·Neuron·E A ErtelW A Catterall
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·D McHughW A Catterall
Oct 23, 2002·The Journal of Cell Biology·Johannes C LenzMichael Schaefer
Nov 20, 2003·The Journal of Biological Chemistry·Ganesan L KamatchiJulianne J Sando
Dec 6, 2003·Journal of Neurophysiology·Kausalia VijayaragavanMohamed Chahine
Mar 6, 2004·Journal of Bioenergetics and Biomembranes·Keith S Elmslie
Jan 13, 2006·British Journal of Pharmacology·Annette C Dolphin
Jul 1, 2006·Biochimica Et Biophysica Acta·Senena Corbalán-García, Juan C Gómez-Fernández

❮ Previous
Next ❯

Citations

Sep 26, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jagmohan SinghSatish Rattan
Apr 9, 2017·Archives of Biochemistry and Biophysics·Senthilkumar RajagopalGanesan L Kamatchi
Apr 7, 2009·Cell Calcium·Damian B van Rossum, Randen L Patterson
Dec 25, 2009·Basic & Clinical Pharmacology & Toxicology·Senthilkumar RajagopalGanesan L Kamatchi
Sep 25, 2015·Journal of Neurophysiology·Jette Skov AlstromNanna MacAulay
Mar 1, 2016·Molecules and Cells·Jin-Young JeongByung-Chang Suh
Aug 2, 2011·Journal of Molecular and Cellular Cardiology·E P ScholzE Zitron

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.