PMID: 7536806May 1, 1995Paper

Protein kinase C modulates calcium channels in isolated presynaptic nerve terminals of rat hippocampus

Journal of Neurochemistry
D K Bartschat, T E Rhodes

Abstract

Nerve terminals ("synaptosomes") isolated from rat brain hippocampus were loaded with the fluorescent Ca2+ indicator fura-2 and were subjected to depolarization with an elevated K+ concentration in a stopped-flow spectrophotometer to measure the activity of voltage-gated Ca2+ channels in the presynaptic membrane. Three components of Ca2+ influx were seen, which were tentatively identified as two classes of voltage-dependent Ca2+ channels with different inactivation kinetics (tau of approximately 60 ms and 1 s, respectively) and Na+/Ca2+ exchange working in the "reverse" mode. The activity of both classes of voltage-dependent Ca2+ channels was slightly augmented by the phorbol ester phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C (PKC), but the effect of PMA was markedly enhanced by the protein phosphatase inhibitor okadaic acid (OKA). The PKC inhibitors calphostin C and dihydrosphingosine (DHS) caused a prompt decrease in voltage-dependent Ca2+ channel activity, but the effect of DHS could be slowed by coaddition of OKA. These results suggest that the activity of presynaptic voltage-dependent Ca2+ channels in the hippocampus is under a dynamic balance between PKC phosphorylation (leading to activation) a...Continue Reading

Citations

Aug 23, 2008·Neuroscience and Behavioral Physiology·A B UzdenskiiA V Lobanov
Mar 3, 2007·Aging Cell·Mark P Mattson
Jan 15, 2002·American Journal of Physiology. Cell Physiology·Robert ZannerChristian Prinz
Nov 6, 2009·Journal of Neurophysiology·Chun Yun Chang, Steven Mennerick
Jul 3, 1999·Physiological Reviews·A MeirR Rahamimoff
Dec 5, 1997·Brain Research. Molecular Brain Research·F GuglielmettiC Bendotti
Mar 15, 2001·Neurochemistry International·S Zagulska-SzymczakL Kaczmarek
Jul 10, 2003·Journal of Photochemistry and Photobiology. B, Biology·Denis E BraginAnatoly B Uzdensky
Apr 30, 2009·The International Journal of Biochemistry & Cell Biology·Dongmin ShaoMustafa B A Djamgoz

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