Protein kinase C regulates amino acid transporter ATB(0,+)

Biochemical and Biophysical Research Communications
Lukasz SamlukKatarzyna A Nałecz

Abstract

ATB(0,+) (SLC6A14) is a transporter specific towards neutral and cationic amino acids, known to be up-regulated in malignant tumor cells. We cloned cDNA for rATB(0,+) and expressed it in HEK 293 cells. The ATB(0,+) over-expression correlated with increased l-leucine transport, stimulated by protein kinase C (PKC) activator and attenuated by PKC inhibitors. Transport stimulation was correlated with phosphorylation on serine moiety of the transporter and its augmented plasma membrane presence. Immunoprecipitation experiments demonstrated ATB(0,+) interaction with PKCα, but not with other classical or novel PKC isoforms. Immunocytochemistry experiments showed a transfer of PKCα to plasma membrane upon phorbol ester activation and co-localization with ATB(0,+). The observed regulation of ATB(0,+) by PKC correlates with high activity of both proteins reported for cancer cells.

References

Jan 1, 1986·The International Journal of Biochemistry·J W Sparks, D L Brautigan
Dec 1, 1995·The Journal of Biological Chemistry·A C Newton
Jan 26, 1995·Biochimica Et Biophysica Acta·J GomezaC Aragón
Feb 16, 2002·Biochemical and Biophysical Research Communications·Takahiro HatanakaVadivel Ganapathy
Apr 30, 2003·Pflügers Archiv : European journal of physiology·Nian-Hang ChenMichael W Quick
Oct 9, 2007·The Biochemical Journal·Enrique Fernández-SánchezFrancisco Zafra
Mar 18, 2008·The Biochemical Journal·Amparo FornésBeatriz López-Corcuera
Sep 6, 2008·Biochemical and Biophysical Research Communications·Magdalena CzeredysKatarzyna A Nałecz
Apr 2, 2011·The Journal of Biological Chemistry·Sonja SucicMichael Freissmuth

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Citations

Dec 7, 2013·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Adrienne MüllerStefanie D Krämer
Jun 25, 2015·BioMed Research International·Alan R PenheiterStephanie K Carlson
Aug 10, 2016·Neurochemical Research·Katarzyna A Nałęcz
Nov 17, 2019·Mathematical Biosciences and Engineering : MBE·Yan LiYao Hua Fan
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Katarzyna A Nałęcz

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