Protein kinase C subtypes and retinal ischemic preconditioning.

Experimental Eye Research
John C DreixlerSteven Roth

Abstract

The purpose of our study was to determine the specific subtypes of protein kinase C involved in the neuroprotection afforded by retinal ischemic preconditioning (IPC), their relationship to the opening of mitochondrial KATP (mKATP) channels, and their role in apoptosis after preconditioning and ischemia. Rats were subjected to retinal ischemia after IPC, or retinas were rendered ischemic after pharmacological opening of mKATP channels. Using immunohistochemistry and image analysis, we determined cellular localization of PKC subtypes. We blocked PKC-delta and -epsilon to study the effect on protection with IPC or with IPC-mimicking by the opening of mKATP channels. PKC subtypes were inhibited pharmacologically or with interfering RNA. Electroretinography assessed functional recovery after ischemia. IPC was effectively mimicked by injection of diazoxide to open the mKATP channel. IPC and/or its mimicking were attenuated by the PKC-delta inhibitor rottlerin and by interfering RNA targeting PKC-delta or -epsilon. Using TUNEL staining and Western blotting for caspase-3 and fodrin breakdown we assessed apoptosis. The injection of interfering RNA to PKC-delta and -epsilon before preconditioning significantly enhanced TUNEL staining as...Continue Reading

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Citations

May 17, 2011·Journal of Molecular Neuroscience : MN·John C DreixlerSteven Roth
Aug 13, 2013·The Journal of Surgical Research·Nari Yun, Sun-Mee Lee
Apr 20, 2011·Experimental Eye Research·John C DreixlerSteven Roth
Nov 26, 2010·Experimental Eye Research·John C DreixlerSteven Roth
Jul 28, 2009·Experimental Eye Research·John C DreixlerSteven Roth
Dec 17, 2008·Experimental Eye Research·John C DreixlerSteven Roth
Mar 27, 2009·Anesthesiology·John C DreixlerSteven Roth
May 20, 2017·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Biji MathewSteven Roth
Nov 28, 2017·Cellular and Molecular Neurobiology·Eric J KnottNicolas G Bazan
Apr 5, 2017·Journal of the Saudi Heart Association·Ahsas Goyal, Neetu Agrawal

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