Protein kinase C theta is dispensable for suppression mediated by CD25+CD4+ regulatory T cells

PloS One
Kerstin SiegmundGottfried Baier

Abstract

The activation of conventional T cells upon T cell receptor stimulation critically depends on protein kinase C theta (PKCθ). However, its role in regulatory T (Treg) cell function has yet to be fully elucidated. Using siRNA or the potent and PKC family-selective pharmacological inhibitor AEB071, we could show that murine Treg-mediated suppression in vitro is independent of PKCθ function. Likewise, Treg cells of PKCθ-deficient mice were fully functional, showing a similar suppressive activity as wild-type CD25+CD4+ T cells in an in vitro suppression assay. Furthermore, in vitro-differentiated wild-type and PKCθ-deficient iTreg cells showed comparable Foxp3 expression as well as suppressive activity. However, we observed a reduced percentage of Foxp3+CD25+ CD4+ T cells in the lymphatic organs of PKCθ-deficient mice. Taken together, our results suggest that while PKCθ is involved in Treg cell differentiation in vivo, it is dispensable for Treg-mediated suppression.

References

Dec 12, 2001·Nature Immunology·Marc A GavinAlexander Rudensky
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Mar 4, 2003·Nature Immunology·Roli KhattriFred Ramsdell
Jun 5, 2003·The Journal of Experimental Medicine·Christa PfeifhoferGottfried Baier
Jul 21, 2004·The Journal of Experimental Medicine·Benjamin J MarslandManfred Kopf
Aug 17, 2004·Immunity·Chyi-Song HsiehAlexander Y Rudensky
Nov 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shahram Salek-ArdakaniMichael Croft
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shahram Salek-ArdakaniMichael Croft
Feb 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Seng-Lai TanSongqing Na
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marina von EssenCarsten Geisler
Aug 2, 2006·Immunity·Rafal PacholczykLeszek Ignatowicz
Aug 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Somia P HickmanLaurence A Turka
Dec 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew A BurchillMichael A Farrar
Mar 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benjamin J MarslandManfred Kopf
Jan 18, 2008·Immunity·Chan-Wang Joaquim Lio, Chyi-Song Hsieh
May 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stephan SauerMatthias Merkenschlager
Apr 10, 2009·Molecular Immunology·Thomas GruberGottfried Baier
May 12, 2009·Nature Immunology·Jhoanne L BautistaChyi-Song Hsieh
Jun 6, 2009·The Journal of Pharmacology and Experimental Therapeutics·Jean-Pierre EvenouGottfried Baier
Sep 10, 2009·The Journal of Experimental Medicine·Monica W L LeungJuan J Lafaille
Feb 5, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mariana I ChuckWeiguo Zhang
Mar 27, 2010·Science·Alexandra Zanin-ZhorovMichael L Dustin
Sep 15, 2010·The Journal of Biological Chemistry·Shudan ShenWeiguo Zhang
Oct 19, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tessy IypeUlrike Lorenz
Feb 4, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kerstin SiegmundJean Pieters
May 25, 2011·The Journal of Experimental Medicine·Amy E MoranKristin A Hogquist
Jan 10, 2012·Annual Review of Immunology·Steven Z JosefowiczAlexander Y Rudensky
Apr 28, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jian MaZuoming Sun
May 16, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Myung-Ja KwonZuoming Sun
Apr 8, 2014·Nature Immunology·Kok-Fai KongAmnon Altman
Apr 12, 2014·Nature Reviews. Immunology·Fred Ramsdell, Steven F Ziegler
May 3, 2014·PloS One·Katarzyna WachowiczGottfried Baier
Sep 30, 2014·Nature Immunology·Andrew G LevineAlexander Y Rudensky
Dec 4, 2014·Immunity·J Christoph VahlMarc Schmidt-Supprian

❮ Previous
Next ❯

Citations

May 30, 2019·Cell Communication and Signaling : CCS·Kerstin SiegmundGottfried Baier
Nov 7, 2019·Cell Communication and Signaling : CCS·Nikolaus ThuilleGottfried Baier
Oct 27, 2018·Frontiers in Immunology·Francesca FerrandinoMaria Pia Felli
Apr 2, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hyunju OhSankar Ghosh

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
FCS
transfection
PCR
electrophoresis
dot blots
FACS

Software Mentioned

GraphPad
GraphPad Prism
FlowLogic

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.