Protein kinase Cepsilon binds peripherin and induces its aggregation, which is accompanied by apoptosis of neuroblastoma cells

The Journal of Biological Chemistry
Lovisa SunessonChrister Larsson

Abstract

A hallmark of the afflicted nervous tissue in amyotrophic lateral sclerosis is the presence of protein aggregates, which to a large extent contain the intermediate filament protein peripherin. Here we show that activation of protein kinase C (PKC) or overexpression of PKCepsilon induces the aggregation of peripherin in cultured neuroblastoma cells with elevated amounts of peripherin. The formation of aggregates was coupled to an increased apoptosis, suggesting a functional link between these events. Both induction of aggregates and apoptosis were suppressed in cells that had been transfected with small interfering RNAs targeting PKCepsilon. PKCepsilon and peripherin associate as shown by co-immunoprecipitation, and the interaction is dependent on and mediated by the C1b domain of PKCepsilon. The interaction was specific for PKCepsilon since corresponding structures from other isoforms did not co-precipitate peripherin, with the exception for PKCeta and -, which pulled down minute amounts. PKCepsilon interacts with vimentin through the same structures but does not induce its aggregation. When the PKCepsilon C1b domain is expressed in neuroblastoma cells together with peripherin, both phorbol ester-induced peripherin aggregation ...Continue Reading

References

Sep 1, 1992·Journal of Neuropathology and Experimental Neurology·M Corbo, A P Hays
Jan 1, 1983·Developmental Neuroscience·M M PortierF Gros
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·M E RippsJ W Gordon
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Jun 26, 1998·The Biochemical Journal·H Mellor, P J Parker
Apr 21, 1999·International Journal of Cancer. Journal International Du Cancer·R ZeidmanC Larsson
Nov 25, 2000·Journal of the Neurological Sciences·J P Julien, J M Beaulieu
Feb 22, 2003·Biochimie·Janice RobertsonJean-Pierre Julien
Mar 19, 2003·The Journal of Cell Biology·Janice RobertsonWalter E Mushynski
Dec 17, 2003·Journal of the Neurological Sciences·Cui Zhen He, Arthur P Hays
May 11, 2004·The Journal of Cell Biology·J M BeaulieuJ P Julien
Aug 24, 2004·The Journal of Biological Chemistry·François Gros-LouisJean-Pierre Julien
Sep 15, 2004·The Journal of Biological Chemistry·Christina SundbergUlla M Wewer
Sep 28, 2004·Brain Pathology·Conrad L LeungArthur P Hays
Feb 22, 2005·Neoplasia : an International Journal for Oncology Research·Jeanette D WaltonRobert A Ross
Aug 18, 2005·Journal of Neuropathology and Experimental Neurology·Michael J StrongHarish C Pant
Nov 5, 2005·The EMBO Journal·Johanna IvaskaPeter J Parker
Mar 1, 2006·Biochimica Et Biophysica Acta·Francois Gros-LouisGuy A Rouleau
Jun 22, 2006·Journal of Neurochemistry·Stéphanie MillecampsJean-Pierre Julien
Jul 25, 2006·Biochimica Et Biophysica Acta·Francheska Colón-González, Marcelo G Kazanietz
Oct 19, 2006·Biochimica Et Biophysica Acta·Shangxi XiaoJanice Robertson
Jul 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Arie GruzmanJian Liu

❮ Previous
Next ❯

Citations

Apr 2, 2011·The Journal of Biological Chemistry·HongBin WangMarcelo G Kazanietz
Feb 19, 2010·Molecular Biology of the Cell·Hongbin Wang, Marcelo G Kazanietz
Nov 19, 2011·Clinical and Experimental Pharmacology & Physiology·Yingxue SuBanghao Zhu
Jul 10, 2009·The Journal of Clinical Investigation·Ronald K H Liem, Albee Messing
May 7, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Virpi TalmanAlessia Pascale
Mar 31, 2010·The Biochemical Journal·Philip M Newton, Robert O Messing
Dec 20, 2014·Cellular Signalling·Bingyu RenYuan Chen
May 1, 2021·Journal of Immunology Research·José Rodrigues do Carmo NetoMarcos Vinícius da Silva

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis