Protein kinase CK2 is coassembled with small conductance Ca(2+)-activated K+ channels and regulates channel gating

Neuron
Wolfgang BildlBernd Fakler

Abstract

Small conductance Ca(2+)-activated K+ channels (SK channels) couple the membrane potential to fluctuations in intracellular Ca2+ concentration in many types of cells. SK channels are gated by Ca2+ ions via calmodulin that is constitutively bound to the intracellular C terminus of the channels and serves as the Ca2+ sensor. Here we show that, in addition, the cytoplasmic N and C termini of the channel protein form a polyprotein complex with the catalytic and regulatory subunits of protein kinase CK2 and protein phosphatase 2A. Within this complex, CK2 phosphorylates calmodulin at threonine 80, reducing by 5-fold the apparent Ca2+ sensitivity and accelerating channel deactivation. The results show that native SK channels are polyprotein complexes and demonstrate that the balance between kinase and phosphatase activities within the protein complex shapes the hyperpolarizing response mediated by SK channels.

References

Sep 1, 1995·European Journal of Biochemistry·V WilhelmJ E Allende
Feb 20, 1999·The Journal of Biological Chemistry·C M FangerJ Aiyar
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·M StockerP Pedarzani
Nov 26, 1999·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·W A Yuhas, P A Fuchs
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·J LudwigB Fakler
Nov 26, 2002·Nature·Jui-Ming LinRavi Allada
Jan 16, 2003·Oncogene·Nathalie Theis-FebvreVéronique Baldin
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Flavio Meggio, Lorenzo A Pinna
Jun 11, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chris T BondJohn P Adelman

❮ Previous
Next ❯

Citations

Sep 10, 2010·Amino Acids·Daniel Bloch HansenIan Henry Lambert
May 20, 2009·Journal of the Association for Research in Otolaryngology : JARO·Julián TarandaA Belén Elgoyhen
Aug 6, 2009·Cell Biochemistry and Biophysics·E S Louise Faber
Jun 1, 2005·Current Opinion in Neurobiology·Chris T BondJohn P Adelman
Jan 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Weiyan Li, Richard W Aldrich
Jul 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Catrin Swantje MüllerUwe Schulte
Mar 1, 2008·The Journal of Biological Chemistry·Tanja BachhuberKarl Kunzelmann
Nov 10, 2011·Molecular & Cellular Proteomics : MCP·Wolfgang BildlUwe Schulte
Feb 17, 2006·Molecular Biology of the Cell·Jinghua HuMaureen M Barr
Jun 7, 2007·Learning & Memory·Laurence RisEmile Godaux
Aug 24, 2007·Clinical and Experimental Pharmacology & Physiology·E S Louise Faber, Pankaj Sah
Dec 5, 2008·American Journal of Physiology. Cell Physiology·Hélène KleinRémy Sauvé
Apr 24, 2009·Journal of Neurophysiology·Max Kleiman-WeinerJohn R Huguenard
Apr 4, 2009·Physiological Reviews·Shuiping DaiJohannes W Hell
Jan 24, 2013·Investigative Ophthalmology & Visual Science·Françoise HaeseleerAmy Lee
Mar 31, 2011·PloS One·Simon M BousquetGuylain Boulay
Oct 15, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amor BelmeguenaiChristian Hansel
Nov 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amy LeeFrançoise Haeseleer
Mar 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Miao ZhangJi-Fang Zhang
Aug 12, 2014·Nature Chemical Biology·Miao ZhangJi-Fang Zhang
Mar 13, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Andrea Hetzel, J Amiel Rosenkranz
Sep 29, 2011·Annual Review of Physiology·John P AdelmanPankaj Sah
Feb 25, 2006·Nature Neuroscience·Irwin B Levitan
May 27, 2014·Journal of Molecular Biology·Alvaro VillarroelPilar Areso
Apr 28, 2011·The Journal of Physiology·Eric HosyChristian Hansel
Dec 22, 2007·The Journal of Physiology·Brenda L Bloodgood, Bernardo L Sabatini
Mar 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·David Nobuhiro DoudaNades Palaniyar
May 6, 2015·Channels·John P Adelman
Mar 15, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Shinkyu ChoiSuk Hyo Suh
Dec 29, 2010·Hearing Research·Eric Wersinger, Paul Albert Fuchs
Oct 12, 2010·Seminars in Cell & Developmental Biology·Uwe SchulteBernd Fakler
Apr 2, 2015·Journal of Cardiovascular Pharmacology·Jonas G DinessMorten Grunnet
May 29, 2009·The European Journal of Neuroscience·Huifang JiPaul D Shepard
Jun 2, 2006·Electrophoresis·Michael BeckerHans Gerd Nothwang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.