Protein kinases and adherens junction dynamics in the seminiferous epithelium of the rat testis

Journal of Cellular Physiology
Nikki P Y Lee, C Yan Cheng

Abstract

Earlier studies in multiple epithelia have shown that cell-cell actin-based adherens junction (AJ) dynamics are regulated, at least in part, by the interplay of kinases and phosphatases that determines the intracellular phosphoprotein content. Yet it is virtually unknown regarding the role of protein kinases in Sertoli-germ cell AJ dynamics in the seminiferous epithelium of the testis. To address this issue, an in vitro coculture system utilizing Sertoli and germ cells was used to study the regulation of several protein kinases, including c-Src (the cellular form of the v-src transforming gene of Rous Sarcoma virus, RSV), carboxyl-terminal Src kinase (Csk), and casein kinase 2 (CK2), during AJ assembly. Both Sertoli and germ cells were shown to express c-Src, Csk, and CK2 with a relative Sertoli:germ cell ratio of approximately 1:1, suggesting both cell types contributed equally to the pool of these kinases in the epithelium. c-Src and Csk were shown to be stage-specific proteins during the epithelial cycle, being highest at stages VII-VIII. Studies using immunoprecipitation have illustrated that these kinases were structurally associated with the N-cadherin/beta-catenin, but not the nectin/afadin, protein complex, implicating ...Continue Reading

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Citations

Jun 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michelle W M LiC Yan Cheng
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·William H Walker
Jun 1, 2005·Development, Growth & Differentiation·Myung Chan GyeYoung Soo Kim
May 17, 2008·Pharmacological Reviews·Dolores D MrukC Yan Cheng
Nov 1, 2011·Pharmacological Reviews·C Yan Cheng, Dolores D Mruk
May 14, 2010·Reproductive Biology and Endocrinology : RB&E·Zhao-juan YangJi Wu
Jul 4, 2009·Endocrinology·Ilona A KoperaDolores D Mruk
Sep 15, 2012·Journal of Cell Science·Xiang XiaoDolores D Mruk
Aug 1, 2012·Journal of Signal Transduction·Cristina BertocchiRonen Zaidel-Bar
Aug 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Helen H N Yan, C Yan Cheng
Jan 26, 2016·Seminars in Cell & Developmental Biology·Pawan Puri, William H Walker
Aug 17, 2011·Molecular and Cellular Endocrinology·A CatizoneM Galdieri
Aug 23, 2006·Journal of Cellular Physiology·John M LukWilliam S B Yeung
Jul 19, 2005·Cytokine & Growth Factor Reviews·Weiliang XiaC Yan Cheng
Dec 11, 2007·Biochimica Et Biophysica Acta·Elissa W P WongC Yan Cheng
Aug 26, 2011·Spermatogenesis·Liza O'DonnellPeter G Stanton
Aug 25, 2015·Molecular and Cellular Endocrinology·Katarzyna Chojnacka, Dolores D Mruk
Dec 24, 2010·The Biochemical Journal·Dolores D Mruk, C Yan Cheng
Oct 20, 2011·The Journal of Toxicological Sciences·Hikari TakahashiShigeru Oshio
Feb 10, 2012·Spermatogenesis·William H Walker
Apr 15, 2014·Seminars in Cell & Developmental Biology·H T WanC Yan Cheng
Feb 18, 2017·Scientific Reports·Christian BorgoLorenzo A Pinna
Jul 25, 2017·American Journal of Veterinary Research·Claire M CannonKhalil Ahmed
Dec 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Helen H N YanC Yan Cheng
Feb 12, 2008·Journal of Cellular Physiology·A CatizoneM Galdieri
Jun 24, 2010·Nature Reviews. Endocrinology·C Yan Cheng, Dolores D Mruk
Sep 1, 2008·Expert Review of Endocrinology & Metabolism·Nikki Py LeeC Yan Cheng

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