Protein kinases and prostaglandin production in ovine astroglia

Prostaglandins
M J NamD W Busija

Abstract

We examined the effects of interleukin-1 alpha (IL-1 alpha) and involvement of protein kinases on prostaglandin production in cultured ovine astroglia. Ovine astroglia were exposed to media alone, or 10 ng/mL IL-1 alpha and prostaglandin F2 alpha (PGF2 alpha) levels were analyzed using enzyme immunoassay. Application of IL-1 alpha augmented the production of PGF2 alpha at 4 h. Coapplication of H-7 (10-1000 microM) and staurosporine (0.1-10 microM), inhibitors of protein kinase C (PKC), blocked IL-1 alpha-induced PGF2 alpha production. IL-1 alpha increased cyclooxygenase (COX) activity while coapplication of staurosporine prevented an increase, implying that COX activity was dependent upon PKC activation. In contrast, forskolin, sodium nitroprusside, and cyclic nucleotide analogs alone did not affect prostaglandin production significantly, excluding the involvement of cAMP/cGMP-dependent protein kinases. Coapplication of quinacrine (10 microM) and bromophenacyl bromide (100 microM), inhibitors of phospholipase A2 (PLA2), prevented the IL-1 alpha-induced increases in PGF2 alpha production. Lastly, IL-1 alpha increased labeled arachidonic acid (AA) release whereas coaddition of quinacrine (10 microM) attenuated increased AA releas...Continue Reading

References

Jan 1, 1979·Annual Review of Physiology·L S Wolfe, F Coceani
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T ShimizuO Hayaishi
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K K WuD S Loose-Mitchell
Mar 1, 1985·Pharmacology, Biochemistry, and Behavior·E B Hollingsworth, G A Patrick
Oct 31, 1988·Biochemical and Biophysical Research Communications·K YamamotoO Hayaishi
Feb 17, 1987·Brain Research·D W Busija, C W Leffler
Nov 30, 1984·Biochemical and Biophysical Research Communications·S Kawamoto, H Hidaka
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Jan 1, 1993·European Journal of Clinical Pharmacology·J PfeilschifterH van den Bosch

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Citations

Oct 29, 2002·FEBS Letters·María A BalboaEdward A Dennis
May 1, 1997·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D W Busija, C Thore
Jun 17, 1998·Stroke; a Journal of Cerebral Circulation·K OsukaJ Yoshida

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