PMID: 6104512Jun 6, 1980Paper

Protein-lipid interaction. Biophysical studies of (Ca2+ + Mg2+)-ATPase reconstituted systems

Biochimica Et Biophysica Acta
J C Gómez-FernándezD Chapman

Abstract

Differential scanning calorimetry, fluorescence spectroscopy and freeze-fracture electron microscopy have been applied to a study of the reconstituted Ca2+-ATPase proteins from sarcoplasmic reticulum when they are incorporated into pure lipid/water systems. The results obtained with these techniques have been used to examine the effects of this intrinsic protein upon the surrounding lipid at temperatures above and below the main lipid solid-fluid phase transition temperature (Tc). 1. Above this Tc value, the freeze-fracture data show that the proteins are randomly distributed within the plane of the bilayer. The fluorescence data show that as the protein content in the bilayer increases, so does the 'microviscosity'. 2. Below Tc the proteins occur in high protein to lipid patches, separate from the remaining crystalline lipid. The fluorescence data indicate that at these temperatures the presence of the protein causes a decrease in microviscosity, whilst the calorimetric data indicate a decrease in enthalpy of the main lipid transition. 3. A premelting of the high protein to lipid patches formed by phase separation within the lipid bilayers is indicated by the calorimetric and fluorescence data. This observation is used to rati...Continue Reading

References

Sep 2, 1975·Biochimica Et Biophysica Acta·D PapahadjopoulosT Isac
Jan 21, 1976·Biochimica Et Biophysica Acta·W Kleemann, H M McConnell
May 2, 1977·Biochimica Et Biophysica Acta·E J Luna, H M McConnell
Dec 1, 1978·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A Seelig, J Seelig
Dec 4, 1978·Biochimica Et Biophysica Acta·E J van ZoelenL L van Deenen
Aug 15, 1977·FEBS Letters·R J CherryG Schneider
May 1, 1975·Quarterly Reviews of Biophysics·D Chapman
Jul 1, 1969·The Journal of Cell Biology·D W Deamer, R J Baskin

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Citations

Jan 1, 1987·CRC Critical Reviews in Biochemistry·C Hidalgo
Mar 1, 1981·European Journal of Biochemistry·W HoffmannD Chapman
Apr 1, 1986·Journal of Bioenergetics and Biomembranes·J A TeruelJ C Gómez-Fernández
Aug 1, 1987·Journal of Bioenergetics and Biomembranes·J A TeruelF Garcia Canovas
Dec 1, 1984·Journal of Bioenergetics and Biomembranes·G SolainiG Lenaz
Sep 20, 2000·The Journal of Biological Chemistry·I Velasco-GuillénJ A Teruel
Jul 27, 2000·Journal of Cellular Physiology·R N KolesnickA Alonso
Jan 1, 1982·The Journal of Membrane Biology·A AlonsoF M Goñi
Jan 19, 2021·Archives of Biochemistry and Biophysics·Alfonso OlivaJosé A Teruel
Nov 1, 1988·Chemistry and Physics of Lipids·T NishiyaT M Chang
Jul 21, 1988·Biochimica Et Biophysica Acta·J M ValpuestaJ M Macarulla
May 15, 1990·Biochimica Et Biophysica Acta·A Villalobo
Aug 24, 1983·Biochimica Et Biophysica Acta·A PradoJ M Macarulla
Jan 11, 1984·Biochimica Et Biophysica Acta·B K SeminM Wikström
Nov 18, 1980·Biochimica Et Biophysica Acta·W HoffmannD Chapman
May 6, 2003·Biochimica Et Biophysica Acta·A G Lee
Dec 1, 1991·Archives of Biochemistry and Biophysics·E del RíoF M Goñi
Apr 29, 1987·Biochemical and Biophysical Research Communications·T NishiyaA Maeda
Aug 8, 2015·ACS Chemical Neuroscience·Alejandro TorrecillasJuan C Gómez-Fernández

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