Protein metabolism in slow- and fast-twitch skeletal muscle during turpentine-induced inflammation

International Journal of Experimental Pathology
Tomas MuthnyMilan Holecek

Abstract

The aim of our study was to evaluate the differences in protein and amino acid metabolism after subcutaneous turpentine administration in the soleus muscle (SOL), predominantly composed of red fibres, and the extensor digitorum longus muscle (EDL) composed of white fibres. Young rats (40-60 g) were injected subcutaneously with 0.2 ml of turpentine oil/100 g body weight (inflammation) or with the same volume of saline solution (control). Twenty-four hours later SOL and EDL were dissected and incubated in modified Krebs-Heinseleit buffer to estimate total and myofibrillar proteolysis, chymotrypsin-like activity of proteasome (CHTLA), leucine oxidation, protein synthesis and amino acid release into the medium. The data obtained demonstrate that in intact rats, all parameters measured except protein synthesis are significantly higher in SOL than in EDL. In turpentine treated animals, CHTLA increased and protein synthesis decreased significantly more in EDL. Release of leucine was inhibited significantly more in SOL. We conclude that turpentine-induced inflammation affects more CHTLA, protein synthesis and leucine release in EDL compared to SOL.

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Citations

Feb 9, 2012·Journal of Physiology and Biochemistry·Miroslav KovarikMilan Holecek
May 9, 2012·International Journal of Experimental Pathology·Milan Holecek
Jun 18, 2017·International Journal of Experimental Pathology·Milan HolecekPavel Siman
Aug 6, 2020·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Esra PekogluSenay Balci
Sep 2, 2020·International Journal of Experimental Pathology·Milan Holeček, Melita Vodeničarovová
Feb 23, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sebastien PreauMervyn Singer
Aug 21, 2010·Journal of Physiology and Biochemistry·Miroslav KovarikMilan Holecek
Nov 13, 2019·Amino Acids·Milan Holeček, Melita Vodeničarovová
Dec 20, 2019·Physiological Research·M Holeček, Vodeničarovová Vodeničarovová
Dec 10, 2020·International Journal of Experimental Pathology·Maria Laura Jorge MichelettoDavid Feder

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