PMID: 3773934Oct 1, 1986Paper

Protein methylation and protein methylases in Leishmania donovani and Leishmania tropica promastigotes

Molecular and Biochemical Parasitology
P PaolantonacciM Robert-Gero

Abstract

We studied the content of acid-stable methylated amino acids of soluble proteins in promastigotes of Leishmania donovani and L. tropica. epsilon-N-Trimethyllysine and NG,NG-dimethylarginine were found in both Leishmania species after culture in the presence of [methyl-14C]methionine. In addition, 3-N-methylhistidine was found only in L. tropica and epsilon-N-dimethyllysine only in proteins of L. donovani. As sinefungin, an antileishmanial nucleoside antibiotic, is a known transmethylase inhibitor, its effect on protein methylation was studied, in whole cells and in vitro. In the first case the drug had no effect on the content of methylated amino acid residues of soluble proteins. In vitro, histone methylation by crude extracts was studied at pH 7.2 and 9.0, known in other organisms as optimum pH values for arginine and lysine methylation, respectively. Surprisingly, arginine methylation by extracts of L. donovani was the same at both pH values while lysine residues were more efficiently methylated at pH 7.2 than at pH 9 by the extracts of the two species. These results indicate that the properties of protein methylases I and III of these parasites are different from those of other organisms hitherto studied. The inhibition con...Continue Reading

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Citations

Jan 1, 1993·Parasitology Research·M A PhelouzatM Robert-Gero
May 9, 1998·Molecular and Biochemical Parasitology·F LawrenceM Robert-Gero
Sep 8, 1999·The Biochemical Journal·M P Hasne, F Lawrence
Sep 1, 1993·The Journal of Eukaryotic Microbiology·F Lawrence, M Robert-Gero
Oct 1, 1987·Antimicrobial Agents and Chemotherapy·L L Nolan
Apr 16, 2018·International Journal of Biological Macromolecules·Pruthvi Raj Bejugam, Shailza Singh
Sep 1, 1987·Biochemical Pharmacology·P PaolantonacciM Robert-Géro
Jun 29, 2021·Frontiers in Molecular Biosciences·Gustavo D CampagnaroPegine B Walrad
May 29, 2007·Journal of Inorganic Biochemistry·Massimo CappannelliGianni Valensin

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