Protein movement across cultured guinea pig trachea: specificity and effect of transcytosis inhibitors

The American Journal of Physiology
M E DeffebachC M Hoy

Abstract

Airway surface liquid (ASL) is a complex fluid with solutes including electrolytes, lipids, mucins, and proteins. The proximal airways are absorptive for most solutes, including proteins. We investigated the process of protein movement across confluent primary cultures of guinea pig trachea grown on filters using fluorescent-labeled bovine serum albumin (BSA), ovalbumin (OA), and 70-kDa dextran (Dex). We found marked asymmetry of BSA and OA transepithelial flux, with apical-to-basolateral flux (JA-->B) 10 times greater than the opposite direction (JB-->A) for both proteins. The apparent permeability for Dex was the same as that for proteins in the basolateral-to-apical direction and showed no asymmetry. Increasing concentrations of unlabeled BSA, OA, or transferrin inhibited JA-->B for both BSA and OA without affecting Dex movement. Cooling reduced JA-->B for BSA without affecting JB-->A. Monensin and nocodazole each reduced JA-->B for BSA and OA without affecting JB-->A. Monensin eliminated all asymmetry for BSA movement. Brefeldin A did not affect JA-->B for either protein but did increase JB-->A for BSA. Treatment with the protease inhibitors increased JA-->B for BSA. Western immunoblotting demonstrated immunologically intac...Continue Reading

Citations

Feb 8, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kwang-Jin KimEdward D Crandall
Mar 9, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Randolph H HastingsMichael A Matthay
Jul 5, 2003·Physiological Reviews·Pamela L Tuma, Ann L Hubbard
Dec 14, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·Francesca BodegaEmilio Agostoni
Oct 17, 2002·Journal of Applied Physiology·Emilio AgostoniLuciano Zocchi
Apr 5, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Joseph ZabnerAloysius J Klingelhutz
Oct 22, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jennifer L SloanSela Mager

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