Protein Network Analysis and Functional Studies of Calcium Oxalate Crystal-Induced Cytotoxicity in Renal Tubular Epithelial Cells

Proteomics
Paleerath PeerapenVisith Thongboonkerd

Abstract

Our previous expression study has reported a set of proteins with altered levels in renal tubular cells after exposure to calcium oxalate monohydrate (COM) crystals, which are the main composition of kidney stones. However, their functional significance remained largely unknown. In this study, protein network analysis revealed that the significantly altered proteins induced by COM crystals were involved mainly in three main functional networks, including i) cell proliferation and wound healing; ii) oxidative stress and mitochondrial function; and iii) cellular junction complex and integrity. Cell proliferation and wound healing assays showed that the COM-treated cells had defective proliferation and tissue healing capability, respectively. Oxyblot analysis demonstrated accumulation of the oxidized proteins, whereas intracellular ATP level was significantly increased in the COM-treated cells. Additionally, level of zonula occludens-1 (ZO-1), a tight junction protein, was significantly decreased, consistent with the significant declines in transepithelial resistance (TER) and level of RhoA signaling molecule in the COM-treated cells. These findings indicate significant perturbations in mitochondrial and oxidative stress axis that...Continue Reading

References

Apr 11, 2002·Urological Research·Ramasamy Selvam
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Karl Matter, Maria S Balda
Jul 4, 2003·Critical Reviews in Eukaryotic Gene Expression·Julie A JonassenCheryl R Scheid
Jun 9, 2005·Journal of Structural Biology·Magali C Chauvet, Rosemary Lyons Ryall
Feb 7, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Visith ThongboonkerdSomchai Chutipongtanate
Oct 15, 2008·Journal of Proteome Research·Visith ThongboonkerdShui-Tein Chen
Jun 23, 2009·Annals of the New York Academy of Sciences·Sandra CitiLaurent Guillemot
Aug 5, 2010·Nature Reviews. Molecular Cell Biology·Stephen W G Tait, Douglas R Green
Sep 22, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Paleerath Peerapen, Visith Thongboonkerd
Nov 3, 2012·Journal of Biomedicine & Biotechnology·Angels Díaz-RamosRoser López-Alemany
Nov 9, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kedsarin Fong-NgernVisith Thongboonkerd
Jan 18, 2017·Metabolism: Clinical and Experimental·Kedsarin Fong-NgernVisith Thongboonkerd
Feb 16, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Siripat AluksanasuwanVisith Thongboonkerd

❮ Previous
Next ❯

Citations

Feb 1, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Supaporn KhamchunVisith Thongboonkerd
Aug 20, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keerakarn SomsuanVisith Thongboonkerd
Jul 9, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hui ZhangJian-Ming Ouyang
Jul 23, 2020·Oxidative Medicine and Cellular Longevity·Zhang YifanJia Ruipeng
Feb 23, 2020·Frontiers in Pharmacology·Visith Thongboonkerd
Jan 8, 2019·Advances in Nutrition·Rattiyaporn Kanlaya, Visith Thongboonkerd
Nov 17, 2020·Frontiers in Physiology·Sakdithep Chaiyarit, Visith Thongboonkerd
Jun 21, 2021·Chemico-biological Interactions·Paleerath Peerapen, Visith Thongboonkerd
Jul 29, 2021·Expert Review of Proteomics·Paleerath Peerapen, Visith Thongboonkerd

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.