Protein partitioning driven by excluded-volume interactions in an aqueous nonionic micellar-gel system

Biotechnology and Bioengineering
D van RoosmalenDaniel Blankschtein

Abstract

The separation and purification of biomolecules in aqueous media driven by excluded-volume interactions is a well-established concept. In this article we propose a new separations method, based on excluded-volume principles, consisting of an aqueous micellar-gel system (AMGS). Specifically, an outer aqueous phase containing cylindrically shaped micelles of the nonionic surfactant n-decyl tetra (ethylene oxide) (C10E4) is physically separated from an inner aqueous phase defined by the interior volume of gel beads, from which the micelles are completely excluded because of their shape and size. In the AMGS, the concentration of the micelles outside the gel beads is sufficiently high that the volume excluded to a biomolecule in the solution external to the gel beads is much larger than that within the gel beads. Accordingly, when biomolecules are introduced into the AMGS, they partition preferentially into the gel-bead phase, according to their sizes, as a result of the greater effect of the excluded-volume interactions with the C10E4 micelles present in the aqueous phase outside the gel beads. The new AMGS is more versatile and adaptable than the conventional two-phase aqueous C10E4 micellar system because the micelle volume frac...Continue Reading

References

Jul 1, 1991·Biotechnology Progress·S H GehrkeN Vaid
Mar 1, 1996·Biophysical Journal·J Tong, J L Anderson
Jul 2, 1998·Biophysical Journal·J C WilliamsS Eichholtz
Aug 12, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·C L LiuD Blankschtein
Jun 13, 2001·Journal of Colloid and Interface Science·Matthew J. LazzaraWilliam M. Deen
Mar 6, 2002·Molecular Biotechnology·Teresa Cunha, Raquel Aires-Barros
Sep 19, 2002·Journal of Chromatography. a·Georges Guiochon
Mar 13, 2003·Biotechnology and Bioengineering·Carlota O Rangel-YaguiDaniel Blankschtein
Jul 25, 2003·Journal of Chromatography. a·Roger M Smith
Oct 20, 1996·Biotechnology and Bioengineering·C L LiuD Blankschtein
Jul 20, 1997·Biotechnology and Bioengineering·T MinuthM R Kula

❮ Previous
Next ❯

Citations

Aug 9, 2013·Biotechnology Progress·João Vitor Dutra MolinoMaria Silvia Viccari Gatti
Dec 6, 2017·Biotechnology and Applied Biochemistry·João Vitor Dutra MolinoAdalberto Pessoa
Sep 30, 2005·Biotechnology and Bioengineering·L J P van den BroekeJ T F Keurentjes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biotechnology and Bioengineering
L J P van den BroekeJ T F Keurentjes
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
D A HornemanL A M van der Wielen
Journal of Chromatography. B, Biomedical Sciences and Applications
C L LiuDaniel Blankschtein
© 2021 Meta ULC. All rights reserved