Protein patterning by microcontact printing using pyramidal PDMS stamps

Biomedical Microdevices
Luisa FilipponiDan V Nicolau

Abstract

Micro-contact printing, μCP, is a well-established soft-lithography technique for printing biomolecules. μCP uses stamps made of Poly(dimethylsiloxane), PDMS, made by replicating a microstructured silicon master fabricated by semiconductor manufacturing processes. One of the problems of the μCP is the difficult control of the printing process, which, because of the high compressibility of PDMS, is very sensitive to minute changes in the applied pressure. This over-sensitive response leads to frequent and/or uncontrollable collapse of the stamps with high aspect ratios, thus decreasing the printing accuracy and reproducibility. Here we present a straightforward methodology of designing and fabricating PDMS structures with an architecture which uses the collapse of the stamp to reduce, rather than enlarge the variability of the printing. The PDMS stamp, organized as an array of pyramidal micro-posts, whose ceiling collapses when pressed on a flat surface, replicates the structure of the silicon master fabricated by anisotropic wet etching. Upon application of pressure, depending on the size of, and the pitch between, the PDMS pyramids, an air gap is formed surrounding either the entire array, or individual posts. The printing tec...Continue Reading

References

May 30, 1997·Science·C S ChenD E Ingber
May 7, 1999·Biosensors & Bioelectronics·D V NicolauS Yoshikawa
Dec 30, 1999·Biomaterials·R S KaneG M Whitesides
Dec 18, 2001·Biosensors & Bioelectronics·Pierre ThiébaudAndreas Offenhäusser
Jul 14, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Kenneth G SharpChung-Yuen Hui
Aug 11, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·M M J DecréR Schroeders
Aug 11, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Yonggang Y HuangAndrew G Alleyne
Sep 6, 2005·Journal of Immunological Methods·Michael AbdoHeddy Zola
Dec 22, 2005·Nature Reviews. Genetics·David B AllisonMahyar Sabripour
Jun 28, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Moniraj GhoshKathleen J Stebe
Jul 8, 2008·The Review of Scientific Instruments·Elie Bou ChakraMichel Cabrera
Aug 16, 2008·Science·Fengwei HuoChad A Mirkin
Nov 4, 2008·Biosensors & Bioelectronics·Luisa FilipponiDan V Nicolau
Sep 19, 2009·Methods in Molecular Biology·Daniel B WolfeGeorge M Whitesides
Jun 1, 2008·Asian Spine Journal·Ki-Won KimYoung-Kyun Woo
Aug 14, 2010·Biosensors & Bioelectronics·Dan V NicolauDuy K Pham
Dec 1, 1998·Analytical Chemistry·D C DuffyG M Whitesides
Nov 30, 2011·Analytical Chemistry·Tohid Fatanat DidarMaryam Tabrizian
Feb 22, 2012·Lab on a Chip·Curtis D ChinSamuel K Sia
Dec 5, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Nikolaos NikogeorgosGraham J Leggett
Jul 11, 2013·Biotechnology Advances·Adnan MujahidAdeel Afzal

❮ Previous
Next ❯

Citations

Oct 19, 2016·Journal of Separation Science·Reinhard I BoysenMilton T W Hearn
Nov 2, 2016·ACS Applied Materials & Interfaces·Saminathan RamakrishnanAdrian Keller
Jan 11, 2018·Chemical Communications : Chem Comm·S M BarteltS V Wegner
Apr 7, 2018·PloS One·Bochao Lu, Michel M Maharbiz
Sep 13, 2017·Macromolecular Bioscience·Vaibhav SharmaElena García-Gareta
Jun 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ran SongChangjian Lin
Jul 17, 2020·Frontiers in Bioengineering and Biotechnology·Sneha GopalJonathan S Dordick
Feb 9, 2019·Frontiers in Chemistry·Marco LindnerEva Sevcsik
Jul 10, 2020·Chemical Society Reviews·Maryam RahmatiHåvard J Haugen
Jun 1, 2019·Stem Cells International·Teresa P SilvaTiago G Fernandes
Oct 2, 2020·Micromachines·Sean BeverungRobert Steward
Jan 5, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Sangwook ChuReza Ghodssi
Sep 17, 2021·Frontiers in Cell and Developmental Biology·Andrew J CapelRussell A Harris

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensors
AFM

Software Mentioned

Pro Plus
SPMLab
WSxM
Image

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Related Papers

Journal of Colloid and Interface Science
Wei ZhangKun-Lin Yang
Methods in Molecular Biology
Yunyan Xie, Xingyu Jiang
© 2022 Meta ULC. All rights reserved