Protein phosphatase 2A activates the HIV-2 promoter through enhancer elements that include the pets site

The Journal of Biological Chemistry
Neil FaulknerDavid M Markovitz

Abstract

Human immunodeficiency virus type 2 (HIV-2) gene expression is regulated by upstream promoter elements, including the peri-Ets (pets) site, which mediate enhancer stimulation following treatment with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). We previously showed that the oncoprotein DEK binds to the pets site in a site-specific manner. In this report, we show that binding to the HIV-2 pets site is modulated by treatment of U937 monocytic cells with TPA, an activator of protein kinase C. TPA treatment resulted in a reduction in the levels of DEK and the formation of a faster migrating pets complex in gel shift assays. We show further that the actions of TPA on pets binding can be duplicated by phosphatase treatment of nuclear proteins and is blocked with okadaic acid, a protein phospatase-2A (PP2A) inhibitor. Finally, we demonstrate that ectopic expression of the catalytic domain of PP2A can activate the HIV-2 enhancer/promoter alone or in synergy with TPA, an effect mediated in part through the pets site. These results suggest that, through an interaction with the protein kinase C pathway, PP2A is strongly involved in regulating HIV-2 enhancer-mediated transcription. This is a consequence of its effects on D...Continue Reading

References

Oct 1, 1992·Baillière's Clinical Haematology·M von LindernG Grosveld
Aug 31, 1992·Biochemical and Biophysical Research Communications·P RieckmannJ H Kehrl
Apr 1, 1991·Molecular and Cellular Biology·S I YangM C Mumby
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D M MarkovitzG J Nabel
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Jul 1, 1993·Experimental Cell Research·M L Seibenhener, M W Wooten
Feb 1, 1993·Annals of Internal Medicine·D M Markovitz
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S DemczukB Vennström
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·G K FuD M Markovitz
Apr 3, 1998·Journal of Virology·R F ChunK T Jeang
Jul 21, 1998·The Journal of Biological Chemistry·Z Baharians, A H Schönthal
Jul 24, 1998·Archives of Biochemistry and Biophysics·R Ricciarelli, A Azzi
Dec 16, 1998·Frontiers in Bioscience : a Journal and Virtual Library·A H Schönthal
May 14, 1999·Trends in Biochemical Sciences·T A MillwardB A Hemmings
Oct 8, 1999·Biochemical and Biophysical Research Communications·S OshevskiM Castagna
Jan 22, 2000·Arthritis and Rheumatism·X DongW H Reeves
Feb 26, 2000·Clinical and Experimental Immunology·I WichmannA Nuñez-Roldan

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Citations

Apr 5, 2011·Cellular and Molecular Life Sciences : CMLS·Fumiaki UchiumiSei-ichi Tanuma
Feb 22, 2003·Nucleic Acids Research·Mónica CampillosJesús Vázquez
Nov 25, 2003·Nucleic Acids Research·Tanja WaldmannClaudia Gruss
Apr 5, 2011·Genes & Development·Ferdinand KappesDavid M Markovitz
Aug 9, 2006·Molecular and Cellular Biology·Trisha M Wise-DraperSusanne I Wells
Oct 13, 2006·Molecular and Cellular Biology·Nirit Mor-VakninDavid M Markovitz
Mar 12, 2008·Molecular and Cellular Biology·F KappesE Ferrando-May
Jun 17, 2004·Molecular and Cellular Biology·Ferdinand KappesTanja Waldmann
Jun 17, 2004·Molecular and Cellular Biology·Ferdinand KappesClaudia Gruss
Feb 19, 2009·Cancer Research·Trisha M Wise-DraperSusanne I Wells
Aug 15, 2009·Cancer Research·Michael S KhodadoustMaría S Soengas
Jun 26, 2003·Arthritis Research & Therapy·Barbara S AdamsDavid M Markovitz
Dec 17, 2014·Viruses·Timsy UppalErle S Robertson
Jul 31, 2009·Science in China. Series C, Life Sciences·Ying HuaXiangLei Peng
Nov 28, 2008·The American Journal of Pathology·Trisha M Wise-DraperSusanne I Wells
Jan 30, 2008·Protein Science : a Publication of the Protein Society·Matthew DevanyHiroshi Matsuo
Jun 5, 2013·Stem Cells·Hal E BroxmeyerDavid M Markovitz
Oct 2, 2015·Journal of Oncology·Nicholas A PeaseLisa Privette Vinnedge
Nov 15, 2002·Biochimica Et Biophysica Acta·Zeping WangNancy S Magnuson
Mar 18, 2017·Current Opinion in Hematology·Maegan L Capitano, Hal E Broxmeyer
Jul 11, 2006·The Journal of Biological Chemistry·Morgan SammonsBrian P Ashburner
Jun 8, 2002·The Journal of Biological Chemistry·Jens ModrofStephan Becker
May 9, 2002·The Journal of Biological Chemistry·Tanja WaldmannClaudia Gruss
Jul 1, 2005·The Journal of Biological Chemistry·Joanne ClearyDavid M Markovitz
Jul 2, 2005·World Journal of Gastroenterology : WJG·Zi-Li LüJian-Ming Wen
Mar 11, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Paola SecchieroGiorgio Zauli

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