Protein phosphatase 2A modulates podocyte maturation and glomerular functional integrity in mice

Cell Communication and Signaling : CCS
Xiujuan ZhuJianhua Mao

Abstract

Protein phosphorylation & dephosphorylation are ubiquitous cellular processes that allow for the nuanced and reversible regulation of protein activity. Protein phosphatase 2A (PP2A) is a multifunction phosphatase that is well expressed in all cell types of kidney during early renal development, though its functions in kidney remains to be elucidated. PP2A conditional knock-out mice was generated with PP2A fl/fl mice that were crossed with Podocin-Cre mice. The phenotype of Pod-PP2A-KO mice (homozygous for the floxed PP2A allele with Podocin-Cre) and littermate PP2A fl/fl controls (homozygous for the PP2A allele but lacking Podocin-Cre) were further studied. Primary podocytes isolated from the Pod-PP2A-KO mice were cultured and they were then employed with sing label-free nano-LC - MS/MS technology on a Q-exactive followed by SIEVE processing to identify possible target molecular entities for the dephosphorylation effect of PP2A, in which Western blot and immunofluorescent staining were used to analyze further. Pod-PP2A-KO mice were developed with weight loss, growth retardation, proteinuria, glomerulopathy and foot process effacement, together with reduced expression of some slit diaphragm molecules and cytoskeleton rearrangeme...Continue Reading

References

Jul 1, 1995·Kidney International·J SvennilsonA Aperia
Dec 22, 1999·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·J SvennilsonA Aperia
Dec 14, 2002·Genesis : the Journal of Genetics and Development·Marcus J MoellerLawrence B Holzman
May 22, 2004·Journal of the American Society of Nephrology : JASN·Thomas Benzing
Jan 22, 2005·Current Opinion in Genetics & Development·Veerle JanssensChristine Van Hoof
Nov 4, 2006·The Journal of Biological Chemistry·Takeshi UchiumiKimitoshi Kohno
Apr 26, 2007·Kidney International·S J ShanklandP Mundel
Aug 5, 2008·Current Medicinal Chemistry·Juan Antonio MorenoAlberto Ortiz
Dec 17, 2010·Cancer Research·Anna A SablinaWilliam C Hahn
May 25, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lydia HanssenUte Raffetseder
Oct 15, 2011·Genesis : the Journal of Genetics and Development·Pengyu GuXiang Gao
Jul 23, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lydia HanssenUte Raffetseder
Dec 18, 2013·Journal of Cellular Biochemistry·Xia JinLizhong Du
Feb 18, 2014·The Journal of Clinical Investigation·Xuefei TianShuta Ishibe
Jun 21, 2014·The Journal of Biological Chemistry·Christina AlidoustyUte Raffetseder
Mar 1, 2015·Biochimie·Sandeep Kumar, Kulbhushan Tikoo
Apr 14, 2015·Cancers·Maeve Kiely, Patrick A Kiely
May 28, 2015·Journal of the American Society of Nephrology : JASN·Wei DongBerend Isermann
Dec 18, 2015·Methods in Molecular Biology·Takahiro HorinouchiSoichi Miwa
Sep 4, 2016·Kidney International·Jialin WangUte Raffetseder
Nov 12, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shi-Wen JiangJiayin Liu
Jul 1, 2017·Journal of Cellular and Molecular Medicine·Jialin WangUte Raffetseder
Sep 3, 2017·Journal of the American Society of Nephrology : JASN·Christoph Schell, Tobias B Huber
Jan 13, 2018·Journal of the American Society of Nephrology : JASN·An S De VrieseFernando C Fervenza
Jul 19, 2018·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Lydia GibbertUte Raffetseder

❮ Previous
Next ❯

Citations

Sep 21, 2021·Experimental and Therapeutic Medicine·Lishi ShaoLi Yang
Nov 5, 2021·Nature Reviews. Nephrology·Lisa SeufertRoman-Ulrich Müller
Jul 7, 2021·Cell Death Discovery·Yinwu BaoWeiqiang Lin

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
genotyping
PCR
ELISA
Protein Assay
transgenic

Software Mentioned

Andromeda
MaxQuant

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochimica Et Biophysica Acta. Molecular Cell Research
Sara Reynhout, V Janssens
The International Journal of Biochemistry & Cell Biology
Caitlin M O'ConnorGoutham Narla
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
Wei-Jun LiuJian Ding
© 2021 Meta ULC. All rights reserved