Protein phosphatase 2A regulates the p38 signaling pathway to affect the migration of astrocytes

Molecular Medicine Reports
Lijun ZhangSibei Ji

Abstract

The aim of the present study was to investigate the effect and mechanism of protein phosphatase 2A (PP2A) on the migration of astrocytes. The primary astrocytes of neonatal mice were isolated and cultured in vitro, and treated with the PP2A activator D‑erythro‑sphingosine (DES) (activated group) or inhibitor okadaic acid (inhibitory group). The control group was treated with equal amounts of dimethyl sulfoxide. The activity of PP2A in the cells was detected using a commercial kit and the migration of cells was investigated using a Transwell migration assay. The protein expression of p38, phosphorylated (p)‑p38, matrix metalloproteinase (MMP)‑2 and MMP‑9 was detected by western blotting. Cell migration and the protein expression of p38, p‑p38, MMP‑2 and MMP‑9 was also determined following treatment of astrocytes with the p38 signaling pathway inhibitor SB202190 with or without the PP2A activator DES. The results demonstrated that the activity of PP2A in the PP2A inhibitory group was significantly decreased compared with the control group, while that of the PP2A‑activated cells was significantly increased compared with the control. The protein levels of MMP‑2 and MMP‑9 in the PP2A inhibitory group astrocytes were significantly de...Continue Reading

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Methods Mentioned

BETA
Protein Concentration assay
confocal microscopy
electrophoresis

Software Mentioned

SPSS

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