Protein phosphatase CaPpz1 is involved in cation homeostasis, cell wall integrity and virulence of Candida albicans

Microbiology
Csaba ÁdámV Dombrádi

Abstract

The opportunistic pathogen Candida albicans has a single protein phosphatase Z (PPZ) candidate gene termed CaPPZ1, which shows significant allele variability. We demonstrate here that bacterially expressed CaPpz1 protein exhibits phosphatase activity which can be inhibited by recombinant Hal3, a known inhibitor of Saccharomyces cerevisiae Ppz1. Site-directed mutagenesis experiments based on natural polymorphisms allowed the identification of three amino acid residues that affect enzyme activity or stability. The expression of CaPPZ1 in ppz1 S. cerevisiae and pzh1 Schizosaccharomyces pombe cells partially rescued the salt and caffeine phenotypes of the deletion mutants. CaPpz1 also complemented the slt2 S. cerevisiae mutant, which is crippled in the mitogen-activated protein (MAP) kinase that mediates the cell wall integrity signalling pathway. Collectively, our results suggest that the orthologous PPZ enzymes have similar but not identical functions in different fungi. The deletion of the CaPPZ1 gene in C. albicans resulted in a mutant that was sensitive to salts such as LiCl and KCl, to caffeine, and to agents that affect cell wall biogenesis such as Calcofluor White and Congo red, but was tolerant to spermine and hygromycin B...Continue Reading

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Citations

Apr 3, 2014·Applied Microbiology and Biotechnology·Gabor NagyGaspar Banfalvi
Aug 22, 2013·Molecular Microbiology·Cristina MoleroJoaquín Ariño
Jan 20, 2015·Molecular Microbiology·Lizziane K WinkelströterGustavo H Goldman
Mar 12, 2015·The New Phytologist·Yingzi YunZhonghua Ma
Jul 4, 2012·Fungal Genetics and Biology : FG & B·Éva LeiterViktor Dombrádi
Aug 25, 2017·PloS One·Bernadett MárkusViktor Dombrádi
May 7, 2015·G3 : Genes - Genomes - Genetics·Lizziane K WinkelströterGustavo H Goldman
Aug 20, 2017·Scientific Reports·Cristina MoleroJoaquín Ariño
Sep 26, 2020·Scientific Reports·Diego VelázquezJoaquín Ariño
Aug 8, 2019·International Journal of Molecular Sciences·Chunyi ZhangJoaquín Ariño
Oct 23, 2020·International Journal of Molecular Sciences·Carlos CalafíJoaquín Ariño
Apr 28, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Carlos CalafíAntonio Casamayor

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