Protein phosphatases 1 and 2A transiently associate with myosin during the peak rate of secretion from mast cells

Molecular Biology of the Cell
Jeff HolstRussell I Ludowyke

Abstract

Mast cells undergo cytoskeletal restructuring to allow secretory granules passage through the cortical actomyosin barrier to fuse with the plasma membrane and release inflammatory mediators. Protein phosphorylation is believed to regulate these rearrangements. Although some of the protein kinases implicated in this phosphorylation are known, the relevant protein phosphatases are not. At the peak rate of antigen-induced granule mediator release (2.5 min), protein phosphatases PP1 and PP2A, along with actin and myosin II, are transiently relocated to ruffles on the apical surface and a band at the peripheral edge of the cell. This leaves an area between the nucleus and the peripheral edge significantly depleted (3-5-fold) in these proteins. Phorbol 12-myristate 13-acetate (PMA) plus A23187 induces the same changes, at a time coincident with its slower rate of secretion. Coimmunoprecipitation experiments demonstrated a significantly increased association of myosin with PP1 and PP2A at the time of peak mediator release, with levels of association decreasing by 5 min. Jasplakinolide, an inhibitor of actin assembly, inhibits secretion and the cytoskeletal rearrangements. Surprisingly, jasplakinolide also affects myosin, inducing the ...Continue Reading

References

Jul 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N SaharaC Oliver
Feb 1, 1991·Current Opinion in Cell Biology·J R Sellers
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Aug 1, 1995·Immunology Today·E RazinJ Rivera
Oct 1, 1995·The Biochemical Journal·S Wera, B A Hemmings
Jan 1, 1995·Cell Motility and the Cytoskeleton·K KawasugiR I Ludowyke
Nov 1, 1993·Molecular and Cellular Biochemistry·R S MoussaviR S Adelstein
Dec 1, 1996·Current Opinion in Immunology·M A Beaven, R A Baumgartner
Jun 23, 1998·Journal of Muscle Research and Cell Motility·D J HartshorneF Erdödi
Feb 27, 1999·Current Opinion in Cell Biology·A R Bresnick
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·J A ValentijnJ D Jamieson
Feb 29, 2000·The Journal of Biological Chemistry·R I LudowykeA T Sim
Apr 25, 2000·Biochemical and Biophysical Research Communications·T HibiM Nakanishi
Jul 6, 2000·European Biophysics Journal : EBJ·M Oheim, W Stühmer
Aug 18, 2000·The Journal of Biological Chemistry·D B Buxton, R S Adelstein
Mar 21, 2001·Cellular Signalling·E Sontag

❮ Previous
Next ❯

Citations

Jul 23, 2004·Molecular Biology of the Cell·Natalia VereshchaginaLuke Alphey
Jul 1, 2008·Biochimica Et Biophysica Acta·Pieter J A EichhornRené Bernards
Feb 24, 2005·Journal of Microscopy·S H CodyJ K Heath
Dec 14, 2006·European Journal of Immunology·Mirvat El-Sibai, Jonathan M Backer
Apr 22, 2004·Trends in Immunology·Ulrich Blank, Juan Rivera
Jun 23, 2006·Pharmacology & Therapeutics·Alistair T R SimNicole M Verrills
Aug 19, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Csilla CsortosAlexander D Verin
Oct 27, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Emilia LecuonaJacob I Sznajder
Oct 1, 2013·Journal of Leukocyte Biology·Giulia MasiCristina Ulivieri
May 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Keri B SanbornJordan S Orange
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Russell I LudowykeTrevor J Biden

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.