PMID: 3215389Nov 1, 1988Paper

Protein phosphorylation changes in bovine carotid artery smooth muscle during contraction and relaxation

Molecular and Cellular Endocrinology
Y TakuwaH Rasmussen

Abstract

The protein phosphorylation changes associated with the contraction and relaxation of bovine carotid artery smooth muscle were studied using two-dimensional gel electrophoresis of labeled phosphoproteins. Muscle was stimulated with histamine, angiotensin II, 12-deoxyphorbol 13-isobutyrate (DPB) or high extracellular K+. Histamine induced a rapid and sustained contraction which was associated with an early (2 min) phosphorylation of 20 kDa myosin light chain (MLC) and two cytosolic proteins, Nos. 1 and 2, and with the late (60 min) phosphorylation of MLC, two isoelectric variants of desmin and ten other cytosolic proteins. Additionally, there was a decrease in the extent of phosphorylation of two cytosolic proteins, Nos. 9 and 10. Angiotensin II induced a rapid but transient contraction which was associated with the same early (2 min) phosphorylation changes, but with none of the late (60 min) changes. Elevation of the extracellular K+ concentration to 110 mM led to a sustained contraction which was associated with the phosphorylation of MLC and proteins Nos. 1 and 2 at both 2 and 60 min, but none of the other late phase phosphoproteins were seen. Addition of DPB, an activator of protein kinase C, induced a slowly developing but...Continue Reading

References

Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Park, H Rasmussen
May 1, 1986·The New England Journal of Medicine·H Rasmussen
Jan 1, 1986·Methods in Enzymology·A B Borle, K W Snowdowne
Dec 1, 1987·The American Journal of Physiology·Y TakuwaH Rasmussen
Sep 1, 1986·The American Journal of Physiology·M Chatterjee, M Tejada
May 1, 1985·Pflügers Archiv : European journal of physiology·T T DeFeo, K G Morgan
Dec 28, 1984·Biochemical and Biophysical Research Communications·N R Danthuluri, R C Deth
Jan 1, 1981·Annual Review of Physiology·D J Hartshorne, R F Siemankowski
Sep 1, 1983·The American Journal of Physiology·M O AksoyR A Murphy
Jul 1, 1984·Physiological Reviews·H Rasmussen, P Q Barrett
Jul 31, 1984·Biochemical and Biophysical Research Communications·H RasmussenA Scriabine
Jan 1, 1980·Annual Review of Biochemistry·R S Adelstein, E Eisenberg
Mar 1, 1983·The American Journal of Physiology·W T Gerthoffer, R A Murphy
Jan 1, 1982·The American Journal of Physiology·M O AksoyK E Kamm
Oct 1, 1982·Pflügers Archiv : European journal of physiology·J P Morgan, K G Morgan
May 1, 1981·The American Journal of Physiology·S P DriskaR A Murphy
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·H Perry-O'KeefeM Egholm
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Sep 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Graham J C UnderwoodDavid N Thomas

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Citations

Dec 16, 1992·Molecular and Cellular Biochemistry·D ZhaoD L Severson
Jan 1, 1990·Pflügers Archiv : European journal of physiology·M SetoY Sasaki
Mar 14, 1995·European Journal of Pharmacology·K X Yang, J L Black
Apr 17, 1990·Molecular and Cellular Endocrinology·W S Zawalich, H Rasmussen
Oct 5, 2013·Histochemistry and Cell Biology·Daniel L WinterZhenlin Li
Jul 1, 1995·Journal of Vascular Surgery·G WhitneyC Brophy
May 15, 1991·Archives of Biochemistry and Biophysics·M BárányK Bárány
Aug 7, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·I Laher, J H Zhang
Jan 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M SugawaT Asano
May 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Y TakuwaT Asano
Nov 1, 1992·Hypertension·J E Andrea, M P Walsh
Jul 10, 2001·American Journal of Physiology. Cell Physiology·S SakuradaY Takuwa
Mar 17, 1998·Biochemical and Biophysical Research Communications·J Florin-ChristensenJ M Delfino
Jul 20, 1990·Biochimica Et Biophysica Acta·K BárányM Bárány
May 1, 1992·Archives of Biochemistry and Biophysics·M BárányK Bárány
Feb 10, 1999·Journal of Vascular Surgery·C M BrophyA Graham
Feb 1, 1997·Journal of Vascular Surgery·C M BrophyA Beall

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